中华肝脏外科手术学电子杂志Issue(5)：318-322,5.DOI:10.3877/cma.j.issn.2095-3232.2015.05.015
杀伤细胞免疫球蛋白样受体基因在肝癌细胞免疫杀伤中的作用
Effect of killer cell immunoglobulin-like receptor gene in immune killing of hepatoma cells
摘要
Abstract
ObjectiveTo investigate the effect of killer cell immunoglobulin-like receptor (KIR) gene in immune killing of hepatoma cells.MethodsPeripheral blood mononuclear cell (PBMC) and hepatoma cells were co-cultured with different effector-target ratios. The expression of KIR gene family in PBMC, the content to interferon-γ (IFN-γ), the morphological change of hepatoma cell and the cytotoxicity to hepatoma cell by PBMC were observed after the co-incubation with different effector-target ratios. The comparison on cytotoxicity rates was conducted using one-way analysis of variance and LSD-t test.ResultsThe expression of activating KIR gene increased after 12 h of co-culture, but decreased after 24 h of co-culture. The expression of inhibitory KIR gene decreased after 12 h of co-culture. DAP12 maintained high expression all the time. The content of IFN-γ in PBMC decreased with the increase of effector-target ratio and reached the peak at 12 h of co-culture. Hepatoma cells co-cultured with different effector-target ratios were observed with increased chromatin condensation, rising proportion of cells with hemispherical or half moon shape and marginalized nucleus, and stagnant of active cell division. The cytotoxicity rate of effector-target ratio 1∶1, 10∶1 and 50∶1 was (8±3) %, (14±4) % and (32±6) %, respectively, with 50∶1 group significantly higher than 11∶1 and 10∶1 group (LSD-t=5.97, 4.61;P<0.05).ConclusionThe activating KIR gene plays an important role in immune killing of hepatoma cells.关键词
受体,KIR/白细胞,单核/受体,自然杀伤细胞/癌,肝细胞/干扰素γKey words
Receptors, KIR/Leukocytes, mononuclear/Receptors, natural killer cell/Carcinoma, hepatocellular/Interferon-gamma引用本文复制引用
何免,邱小惠,张文峰,沈晗,王辉,薄华本,黄树林,曾宪成,邵红伟..杀伤细胞免疫球蛋白样受体基因在肝癌细胞免疫杀伤中的作用[J].中华肝脏外科手术学电子杂志,2015,(5):318-322,5.基金项目
国家自然科学基金基金（31100664，31300737，81303292）；广东省自然科学基金（10151022401000024，2014A030313586）；广东药学院师资队伍建设专项经费（20080927）；广州市卫生局项目（20141A011117，20151A011112）；广州市科技计划项目 （）
