中华灾害救援医学2015，Vol.3Issue(10)：568-571,4.DOI:10.13919/j.issn.2095-6274.2015.10.009
HPLC测定柿叶提取物中芦丁在大鼠体内的血药浓度及药动学研究
Study on plasma concentration of rutin in extract of persimmon leaves in rats by HPLC and its pharmacokinetics
陈杨芳1
作者信息
- 1. 212200 镇江,江苏省扬中市药品检验所
- 折叠
摘要
Abstract
Objective To establish a high performance liquid chromatography (HPLC) method for measuring the concentration of rutin in extract of persimmon leaves in rats, and to study its pharmacokinetics.Methods Blood sample were collected through venous plexus in rat eye at different time after oral administration of the extract of persimmon leaves. After liquid extraction with methanol, the concentration of rutin in plasma was measured. Then pharmacokinetics parameters were calculated by 3P97 program.Results The results showed that plasma concentration-time curve of rutin in rats was coincided with two-compartment model, rutin was absorbed fast in rats. Peak concentration (Cmax) of rutin in rat plasma was (1.658±0.184) mg/L one hour after oral administration. The other main pharmacokinetic parameters were as follows: absorption rate constant (Ka) was (2.436±0.883)/h, distribution rate constant (α) was (1.002±0.372)/h, elimination rate constant (β) was (0.083±0.067)/h, elimination half time (t1/2β) was (9.418±7.256) h, area under the concentration-versus-time curve (AUC0-12) was (9.628±2.281) mg·h/L.Conclusions HPLC method is accurate and sensitive, it is suitable for the pharmacokinetic study of rutin in extract of persimmon leaves in rats.关键词
芦丁/柿叶提取物/药动学/高效液相色谱法Key words
rutin/extract of persimmon leaves/pharmacokinetics/HPLC分类
医药卫生引用本文复制引用
陈杨芳..HPLC测定柿叶提取物中芦丁在大鼠体内的血药浓度及药动学研究[J].中华灾害救援医学,2015,3(10):568-571,4.
