神经药理学报Issue(4)：6-11,6.
棉籽总黄酮抗抑郁活性及其对海马单胺水平的调节
The Antidepressant Effect of the Total Flavonoids Extracted from Cottonseed and its Regulation on Hippocampal Monoamines
摘要
Abstract
Objective:To explore the antidepressant effect of the total flavonoids extracted from cottonseed (CTN-T) and its possible mechanism. Methods:The learned helplessness model in rats was used to evaluate the antidepressant effect of CTN-T. Using the 5-hydroxytryptophan (5-HTP)induced head twitch response test and yohimbine toxicity potentiation test in mice,the possible monoamine targets of the antidepressant action of CTN-T were detected. Moreover, high performance liquid chromatography and electrochemical detection (HPLC-ECD) was used to determine the content of monoamine and metabolites in the hippocampus of rats following CTN-T administration. Results:Repeated administration of CTN-T (50,100 mg·kg-1,ig,twice per day for 4 d) significantly decreased the helplessness behavior induced by inescapable foot-shock in learned helplessness test in rats. Repeated administration of CTN-T (50,75 mg·kg-1, ig,twice per day for 4 d) significantly increased the 5-HTP induced head twitch response in mice,while CTN-T (60 mg·kg-1,ig,twice per day for 4 d) had a tendency to potentiate yohimbine toxicity in mice. Repeated administration of CTN-T (25~75 mg·kg-1,ig,twice per day for 4 d),the neurochemical assays showed that the content of 5-hydroxytryptamine (5-HT) was increased in rat hippocampus at 25 and 100 mg·kg-1 dose of CTN-T,and that the content of norepinephrine (NE) was increased at 50 and 100 mg·kg-1 dose of CTN-T. By contrast,CTN-T had no effects on the content of dopamine (DA),epinephrine (E),5-hydroxyindolacetic acid (5-HIAA) and the rate of 5-HT/5-HIAA. Conclusion:CTN-T exerts antidepressant effects which may be closely related to the strengthening of the hippocampal serotonergic and noradrenergic function.关键词
抑郁/棉籽总黄酮/获得性无助/5-羟色胺/去甲肾上腺素Key words
depression/total flavonoids extracted from cottonseed(CTN-T)/learned helplessness model/5-hydroxytryptamine(5-HT)/norepinephrine(NE)分类
医药卫生引用本文复制引用
赵楠,张有志,杨明,王伊文,赵毅民,宫泽辉,李云峰..棉籽总黄酮抗抑郁活性及其对海马单胺水平的调节[J].神经药理学报,2011,(4):6-11,6.基金项目
北京市自然科学基金项目(No.7042052),国家高技术研究发展计划(863计划)资助项目(No.2002AA2Z3131) （No.7042052）
