神经药理学报Issue(4)：12-15,4.
连翘苷对MPP诱导人神经母细胞株SH-SY5Y细胞损伤的保护作用
Effects of Phillyrin on MPP+-induced Injury in SH-SY5Y Neuroblastoma Cells
摘要
Abstract
Objective:To investigate the effects of Phillyrin on 1-methyl-4-phenylpyridini-um (MPP+)-induced injury in SH-SY5Y neuroblastoma cells. Methods:SH-SY5Y cells were in-cubated with Phillyrin of different concentrations. MTT metabolic rate was measured as the cell survival rate to determine the effective concentrations of Phillyrin. After SH-SY5Y cells were incubated with Phillyrin (1,10,100μmol·L-1) for 24 h prior to exposure to MPP+(1 mmol·L-1) for 48 h,the MTT metabolic rate and LDH leakage rate were observed. Results:Phillyrin did not decrease the survival rate of SH-SY5Y cells at the concentrations from 1 to 100μmol·L-1. Com-pared with the control group,MPP+ decreased the MTT metabolic rate(P<0.001)and increased the LDH leakage rate (P<0.001) of SH-SY5Y cells. However,Phillyrin significantly increased the cell activity (P<0.05) and decreased LDH leakage rate of SH-SY5Y cells with MPP+-induced injury. Conclusion:Phillyrin protects SH-SY5Y cells against MPP+-induced cytotoxicity.关键词
连翘苷/1-甲基-4-苯基吡啶离子/SH-SY5Y细胞/神经保护Key words
Phillyrin/1-methyl-4-phenylpyridinium/SH-SY5Y cells/neuroprotective分类
医药卫生引用本文复制引用
张美蓉,魏守蓉,吴燕川,孙芳玲,艾厚喜,张丽,王文..连翘苷对MPP诱导人神经母细胞株SH-SY5Y细胞损伤的保护作用[J].神经药理学报,2011,(4):12-15,4.基金项目
科技部国家“十二五”重大新药创制专项(No.2012ZX09102201-106),国家自然科学基金项目(No.81173575、30740053),北京市自然科学基金项目(No.7062031),北京市教委科研基地-科技创新平台(No.111219),北京市卫生系统高层次卫生技术人才(No.2011-3-097) （No.2012ZX09102201-106）
