浙江医学Issue(7)：510-512,515,4.
SKP2和p27kip1在放疗后鼻咽癌CNE2细胞应答中的作用
摘要
Abstract
Objective To investigate the expression of SKP2 and p27kip1 in the cel ular response to radiation in human na-sopharyngeal carcinoma cel s CNE-2. Methods The expressions of SKP2 and p27kip1 in human nasopharyngeal carcinoma CNE-2 cel s were detected by Western blot before and after ionizing radiation. Flow cytometry was used for cel cycle Analysis. Results The CNE2 cel s displayed a G2/M cel-cycle arrest after exposure to radiation. Western blot analysis showed a transient increasd expression of p27kip1 and decreased expression of Skp2 at 0.5~1.5h after exposure to low doses ionizing radiation (2Gy);meanwhile 2~4h after exposure to low dose radiation the expression of Skp2 was up-regulated and p27kip1 down-regulated. However, the expressions pattern of SKP2 and p27kip1 were reversed to above observation after exposure to high doses ionizing radiation (6Gy). Conclusion The results suggest that SKP2 may contribute to the regulation of p27kip1 and may be a key factor in regulating the cel cycle in response to radiation-induced DNA damage.关键词
SKP2/p27kip1/鼻咽癌/放射疗法/细胞周期Key words
SKP2/p27kip1/Nasopharyngeal carcinoma/Radiation/Cel cycle引用本文复制引用
杨伟芳,孙晓南,丁维军..SKP2和p27kip1在放疗后鼻咽癌CNE2细胞应答中的作用[J].浙江医学,2013,(7):510-512,515,4.基金项目
浙江省医药卫生科技计划项目(2009A221) （2009A221）
