浙江医学Issue(12)：1143-1146,4.
人参二醇对鱼藤酮、MPP+诱导的PC12细胞损伤的作用
摘要
Abstract
Objective To evaluate the effects of panoxadiol on rat pheochromocytoma PC12 cel injury induced by rotenone or MPP+. Methods PC12 cel injury was induced by rotenone (0.3, 1, 3, 10, 30μmol/L,24h) or MPP+ (0.1, 0.3, 1, 3 mmol/L, 48h). Panoxadiol (10, 25, 50, 75,100 mg/L) was used to treat rotenone or MPP+induced PC12 cel s. Cel proliferation was assessed by MTT method, cel necrosis and apoptosis was detected by flow cytometry with PI (propidium iodide) and Hoechst 33342 stain, respectively. The expression of tyrosine hydroxylase was measuared via immunohistostaining. Results Panoxadiol (10, 25, 50, 75, 100mg/L) had no affcts on proliferation of PC12 cel s and had no protection effects on cel injury induced by rotenone. When PC 12 cel injury was induced by MPP+, panoxadiol(50,75 mg/L) promoted cel proliferation but had no effects on necrocytosis and apoptosis and the expression of tyrosine hydroxylase. Conclusion Panoxadiol (50、75 mg/L) might have pro-tection effects on PC12 cel injury induced by MPP+, which may be related to facilitation of cel proliferation;however, panoxadiol has no protection effects on cel injury induced by rotenone.关键词
人参二醇/鱼藤酮/MPP+/大鼠/PC12细胞Key words
Panoxadiol/Rotenone/MPP+/Rat/PC12 cell引用本文复制引用
金晓蓉,孙馨,林筱洁,苗青,高瑞兰,方桂伦..人参二醇对鱼藤酮、MPP+诱导的PC12细胞损伤的作用[J].浙江医学,2013,(12):1143-1146,4.基金项目
浙江省卫生厅中医药科技研究项目基金资助 （）
