浙江医学Issue(13)：1238-1241,4.
鼻咽癌组织中p15INK4b和p27KIP1基因甲基化及其相关性研究
Promoter methylation status of p15INK4b and p27KIP1 genes in nasopharyngeal carcinoma
王克义 1倪海峰 2沈俊娅 3冷建杭 4王建莉4
作者信息
- 1. 310058 杭州，浙江大学免疫学研究所
- 2. 杭州市第一人民医院中心实验室
- 3. 杭州市第一人民医院耳鼻咽喉科，中心实验室
- 4. 杭州市第一人民医院中心实验室
- 折叠
摘要
Abstract
Objective To investigate the promoter methylation status of p15INK4b and p27KIP1 genes in nasopharyngeal car-cinoma (NPC). Methods The methylation status of p15INK4b and p27KIP1 genes promoter region were detected by methyla-tion- specific polymerase chain reaction (MSP) in 65 specimens of NPC tissues (NPC group) and 30 specimens of normal na-sopharyngeal epithelia tissues (control group). Results The promoter methylation rates of p15INK4b and p27KIP1 genes were 26.2%(17/65) and 61.5%(40/65), respectively in NPC group , while no methylation of the two genes were detected in control group (P<0.01). The methylation of p15INK4b and p27KIP1 genes were not correlated with the clinicopathological characteristics of patients with NPC (P>0.05). There were no significant correlation between the methylations of p15INK4b genes and p27KIP1 genes (r=- 0.03, P>0.05). Conclusion The methylation of p15INK4b and p27KIP1 genes promoter regions may be associated with the development of NPC, which may used as potential markers for early diagnosis of NPC.关键词
鼻咽癌/p15INK4b基因/p27KIP1基因/甲基化Key words
Nasopharyngeal carcinoma/p15INK4b/p27KIP1/Methylation引用本文复制引用
王克义,倪海峰,沈俊娅,冷建杭,王建莉..鼻咽癌组织中p15INK4b和p27KIP1基因甲基化及其相关性研究[J].浙江医学,2013,(13):1238-1241,4.
