浙江医学Issue(15)：1392-1395,1417,5.
体外扩增人NK细胞及其对白血病细胞杀伤作用的研究
Expansion of human natural killer cells with specific cytotoxicity against leukemia cells in vitro
摘要
Abstract
Objective This study is to investigate the method for expansion of human NK cells in vitro. We also tested the role of expanded NK cells in killing of K562 and HL60 leukemia cells and its mechanism. Methods Peripheral blood mononucle-ar cells (PBMC) were isolated from peripheral blood donored by 5 healthy individuals. PBMC were co- cultured with irradiated genetic modified K562 cel transfectants for 14 days. The cultures were then analyzed for fold expansion and cytotoxicity by flow cytometry and 51Cr- release assays. Results This co- culture system could effectively expand NK cells (by a mean of 202 folds). The expanded NK cells killed K562 and HL60 cells avidly as shown by 51Cr- release assays. The mean cytotoxic rate of expanded NK cel s against leukemia cells was 77.1%(K562), 61.2%(HL60) at an E∶T ratio of 8∶1 compared to 39.0%(K562), 35.4% (HL60) of non- expanded NK cel s, respectively. There was no killing of auto- and al o- PBMC. We observed stronger ex-pression of activating receptor NKG2D, NKp30, NKp44 in expanded NK cel s than in non- expanded NK cells. These specific an-tibodies could partly inhibited the enhanced lysis of K562 cells by expanded NK cells. Conclusion K562 transfectants can stim-ulate vigorous expansion of NK cells, and the expanded NK cells had an enhanced cytotoxic effect against leukemia cells. Up- regulation of activating receptors on expanded NK cells may be the mechanisms of enhanced killing of leukemia cells by ex-panded NK cells. NK cell therapy may be used for immunotherapy of leukemia.关键词
自然杀伤细胞/扩增/白血病Key words
Natural killer cells/Expansion/Leukemia引用本文复制引用
蒋卫,韩颖,吴晓松,吴慧群,施菊妹..体外扩增人NK细胞及其对白血病细胞杀伤作用的研究[J].浙江医学,2013,(15):1392-1395,1417,5.基金项目
国家自然科学基金（81071856、30973450）、上海市浦江人才计划（11PJ1407900）；上海市科委基金（12410705100）；同济大学附属第十人民医院基金 （）
