肿瘤药学Issue(2)：92-95,4.DOI:10.3969/j.issn.2095-1264.2013.021
5,7-DMF 诱导14-3-3σ基因甲基化促进乳腺癌MDA-MB-453细胞凋亡的实验研究★
Research on the Promotion of Apoptosis by 5,7-DMF in Breast Cancer Cells through Inducing the Methylation of 14-3-3σGene
摘要
Abstract
Objective To investigate the effects of 5, 7-DMF on the apoptosis of human breast cancer cells and its possible mechanisms. Methods Human breast cancer MDA-MB-453 cells, MCF-7 cells and human immortalized mammary epi-thelial HBL-100 cells were cultured in vitro. After treated with 5, 7-DMF, the cell viability was measured by MTT assay, and the rate of apoptosis was detected by using flow cytometry with PI staining. The methylation status of 14-3-3σ gene was determined by methlation specific PCR (MSP). The expression of 14-3-3σ protein was analyzed by Western blotting. Re-sults 5,7-DMF showed the strongest activity against MDA-MB-453 cells in vitro. After treated for 48 h, 5,7-DMF (5, 10 and 20 μmol·L-1) markedly induced the apoptosis of MDA-MB-453 cells, and the level of methylated 14-3-3σ gene was significantly increased, while the expression of 14-3-3σ protein was significantly decreased. Conclusion 5,7-DMF could induce the apoptosis of breast cancer MDA-MB-453 cells. Its mechanism may be related with the induction of the methyla-tion of 14-3-3σgene and the decrease of the expression of 14-3-3σprotein.关键词
乳腺癌/5,7-二甲氧基黄酮/凋亡/14-3-3σKey words
Breast cancer/5,7-DMF/Apoptosis/14-3-3σ分类
医药卫生引用本文复制引用
刘胜姿,谭宇婷,刘英姿,刘飞,周源..5,7-DMF 诱导14-3-3σ基因甲基化促进乳腺癌MDA-MB-453细胞凋亡的实验研究★[J].肿瘤药学,2013,(2):92-95,4.基金项目
湖南省卫生厅科研项目资助(No.B2010-058) （No.B2010-058）
