中南民族大学学报（自然科学版）Issue(2)：29-31,45,4.
超滤质谱法研究大黄蒽醌类化合物与人血清白蛋白的相互作用
Study on the Interactions between HSA and Rhubarb Anthraquinone Analogues by Ultrafiltration Mass Spectrometry
摘要
Abstract
To study the interactions between small drug molecules and the target protein, an ultrafiltration technology coupled with liquid chromatography-ESI-mass spectrometry ( LC-ESI-MS) method was established and applied to screen the rhubarb anthraquinone analogues ( chrysophanol, rhein and aloe-emodin ) that interacted with human serum albumin ( HSA) . Comparing the extracted ion chromatography ( EIC) of these drugs before and after the incubation with HSA,the sesults indicated that the binding affinity capacity of rhein was the strongest, followed by aloe-emodin, and chrysophanol was the weakest. This work demonstrated that ultrafiltration mass spectrometry is a fast and sensitive analytical method for the study of interactions of protein-ligand complexes.关键词
超滤质谱/人血清白蛋白/大黄蒽醌类化合物/蛋白质-配体复合物Key words
ultrafiltration mass spectrometry/human serum albumin ( HSA)/rhubarb anthraquinone analogues/protein-ligand complexes分类
化学化工引用本文复制引用
王献,喻花..超滤质谱法研究大黄蒽醌类化合物与人血清白蛋白的相互作用[J].中南民族大学学报（自然科学版）,2013,(2):29-31,45,4.基金项目
国家自然科学基金资助项目(21275167) （21275167）
中南民族大学中央专项重点资助项目(CZZ12003) （CZZ12003）
