作物学报Issue(1)：12-20,9.DOI:10.3724/SP.J.1006.2013.00012
大豆叶面茸毛密度和长度的QTL定位
QTL Mapping of Pubescence Density and Length on Leaf Surface of Soybean
摘要
Abstract
Soybean pubescences are known to play important roles in resistance to pests and tolerance to drought stress. QTL mapping of leaf pubescence density and length was conducted in recombinant inbred line populations of NJRIKY (KY) and NJRIXG (XG). The results obtained were as follows:(1) There existed great variation and certain transgressive segregation in leaf pubescence density and length among lines;highly significant negative correlations (r=−0.49 and−0.62, respectively) between the two traits were observed;the heritability values for pubescence density ranged from 75.7%to 76.8%, higher than that for pu-bescence length ranged from 45.2%to 62.9%in the two populations. (2) Two major QTL for pubescence density detected were PD1-1 accounted for 20.7%of phenotypic variation in XG, and PD12-1 contributed 21.7%of phenotypic variation in KY. The genetic constitution of pubescence density was composed of additive QTL (20.7−36.2%of phenotypic variation), epistatic QTL pairs (0−1.4%) and collective unmapped minor QTL (38.1−56.1%) in the two populations. Here the unmapped minor QTL ac-counted for the most part for the trait, which was not recognized if only using mapping procedures without the consideration of the total genetic variation among the lines. (3) The phenotypic variation of pubescence length in KY was accounted for by epistatic QTL pairs (4.2%) and collective unmapped minor QTL (58.7%) without additive QTL (0%), while that in XG mainly by additive QTL, including PL1-1 and PL12-1 on chromosomes 1 and 12 accounting for 18.3%and 22.5%of phenotypic variation, respectively, with very small contribution by epistatic QTL pair and collective unmapped minor QTL. Therefore, the genetic con-stitutions of pubescence length in the two populations were different from each other. The genetic mechanisms of leaf pubescence density and length in soybean are complicated and involve many genes/QTL with different effects.关键词
大豆/茸毛密度/茸毛长度/QTL定位Key words
Soybean (Glycine max [L.] Merr.)/Pubescence density/Pubescence length/QTL mapping引用本文复制引用
邢光南,刘泽稀楠,谭连美,岳汉,王宇峰,KIM Hyun-Jee,赵团结,盖钧镒..大豆叶面茸毛密度和长度的QTL定位[J].作物学报,2013,(1):12-20,9.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2009CB1184,2010CB1259,2011CB1093),国家自然科学基金项目(30900902,31071442),高等学校博士学科点专项科研基金课题(20090097120017),国家大学生创新性实验计划项目(111030713)和南京农业大学SRT项目(1111A03)资助 （973计划）
