作物学报Issue(3)：423-430,8.DOI:10.3724/SP.J.1006.2013.00423
利用分裂泛素酵母双杂交技术钓取小麦TaSC互作蛋白质
Fishing TaSC Interacting Protein in Wheat Using Split Ubiquitin Yeast Two Hybrid System
摘要
Abstract
The TaSC (Triticum asetivum L. salt-tolerance related gene, GenBank accession number AY956330) has been cloned from wheat salt tolerant mutant RH 8706-49, which is induced by high salinity. To fish interacting proteins of this gene, we used TaSC protein as bait to hybrid with the wheat cDNA expression library using the split ubiquitin yeast two hybrid system in this experiment. A novel wheat gene (GenBank accession number AK336035) encoding a protein with unknown function was isolated, which was designated TaSCIP1 (TaSC interaction protein 1). Bimolecular fluorescence complementation (BiFC) experiment con-firmed the interaction between proteins TaSCIP1 and TaSC. The separation of TaSC-interacting protein is beneficial to disclose the mechanism of salt tolerance gene TaSC.关键词
TaSC/小麦/cDNA表达文库/分裂泛素酵母双杂交/双分子荧光互补技术Key words
TaSC/Triticum asetivum L./cDNA expression library/Split ubiquitin yeast two-hybrid system/BiFC引用本文复制引用
蔺芳芳,杨旭,武小翠,刘晓梅,葛荣朝,赵宝存..利用分裂泛素酵母双杂交技术钓取小麦TaSC互作蛋白质[J].作物学报,2013,(3):423-430,8.基金项目
本研究由国家自然科学基金项目(30871471),河北省自然科学基金项目(C2011205085),河北省自然科学基金基地项目(08B019)和河北师范大学博士基金(L2005B20)资助 （30871471）
