作物学报Issue(3)：431-439,9.DOI:10.3724/SP.J.1006.2013.00431
小麦Mlo反义基因的转化及转基因植株的白粉病抗性分析
Transformation of Antisense Wheat Mlo (Ta-Mlo) Gene and Wheat Powdery Mildew Resistance Analysis of Transgenic Plants
摘要
Abstract
The antisense wheat Mlo gene, Ta-Mlo, was transformed into wheat (Tritivum aestivum L.) varieties Yangmai 158 and Jimai 20 via biolistic transformation using immature embryo calli as explants. After two rounds of bialaphos selection and regen-eration, herbicide-resistant plants were obtained, which were subsequently confirmed by PCR, PCR-Southern hybridization, ge-nomic dot hybridization, and BASTA resistance analysis. The results showed that the Ta-Mlo antisense transgenic Yangmai 158 and Jimai 20 plants were obtained. The real time fluorescence quantitative PCR analysis proved that the transcript of Ta-Mlo was knocked down in these transgenic plants. The disease resistance test showed that the six transgenic lines appeared highly resis-tance to powdery mildew pathogen Blumeria graminis f. sp. tritici (Bgt). The transgenic lines showed distinct acceleration of the production and stabilization of papillae, and effective suppression to further development of haustoria of Bgt. Therefore, the transgenic lines showed high resistance to Bgt.关键词
基因枪转化/小麦反义Mlo基因/小麦白粉病/乳突和吸器Key words
Biolistic transformation/Antisense wheat Mlo gene/Wheat powdery mildew/Papillae and haustoria引用本文复制引用
邢莉萍,钱晨,李明浩,曹爱忠,王秀娥,陈佩度..小麦Mlo反义基因的转化及转基因植株的白粉病抗性分析[J].作物学报,2013,(3):431-439,9.基金项目
本研究由国家自然科学基金项目(31101206),教育部高等学校博士点基金项目(20100097120036),江苏省自然科学基金项目(BK2011636)和山东农业大学作物生物学国家重点实验室开放课题项目(2012KF09)资助 （31101206）
