作物学报Issue(3)：520-529,10.DOI:10.3724/SP.J.1006.2013.00520
甘蓝型油菜黄化突变体的光合特性及叶绿素荧光参数分析
Photosynthetic Characteristics and Chlorophyll Fluorescence Kinetic Parame-ters Analyses of Chlorophyll-Reduced Mutant in Brassica napus L.
摘要
Abstract
We investigated the photosynthetic pigment contents, photosynthetic characteristics, chlorophyll fluorescence kinetic parameters and agronomic traits at five-leaf stage of the chlorophyll-reduced mutant (NY), wild-type (NG), F1, and rF1 of their combinations (reciprocal cross). The results showed that the chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids in the mutant were significantly reduced compared with those in other materials, especially for chlorophyll b. The net photosynthetic rate (Pn) of the mutant was significantly lower than those of wild-type and their F1 and rF1. Relatively high intercellular CO2 con-centration (Ci) and an equivalent stomatal conductance (Gs) in the mutant indicated that stomatal factor was not the limiting factor of the photosynthetic rate. The mutant had higher light compensation point (LCP) and light saturation point (LSP), and its dark respiration rate (Rd) was equal to that of wild-type, whereas apparent quantum yield (AQY) and quantum yield at light compensa-tion point (Φc) in the mutant were significantly decreased. The CO2 compensation point (CCP), rate of photorespiration (Rp) and carboxylation efficiency (CE) of the mutant NY were significantly lower than those of the wild-type NG and their combinations of NY×NG and NG×NY, but the mutant had higher CO2 saturation point (CSP). The fluorescence parameters in the mutant, includ-ing Fo, Fm, Fv/Fm, Fv'/Fm',ΦPSI , qp, NPQ, and ETR were significantly reduced. Lower photosynthetic pigment content may be the main reason for low leaf photosynthetic rate which directly leads to the lower light-harvesting capacity and photochemical con-version efficiencies of the PSII reaction center. Moreover, lasting etiolation of leaves in the mutant had a greater impact on its growth and development, but the photosynthetic characteristics and agronomic traits were restored to normal level in F1 of the cross between the mutant and the normal parent.关键词
甘蓝型油菜/黄化突变体/光合色素含量/光合特性/叶绿素荧光动力学参数Key words
Brassica napus L./Chlorophyll-reduced mutant/Photosynthetic pigment content/Photosynthetic characteristics/Chlorophyll fluorescence kinetic parameters引用本文复制引用
肖华贵,杨焕文,饶勇,杨斌,朱英..甘蓝型油菜黄化突变体的光合特性及叶绿素荧光参数分析[J].作物学报,2013,(3):520-529,10.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2011AA10A104)和国家现代农业产业技术体系建设专项(CARS-13)资助 （863计划）
