作物学报Issue(12)：1899-1905,7.DOI:10.3724/SP.J.1006.2015.01899
四倍体小麦地方品种矮蓝麦矮秆性状的遗传分析
Genetic Analysis on Dwarfing Trait in Landrace Ailanmai of Triticum turgidum L. ssp. turgidum
摘要
Abstract
Ailanmai is an important Triticum turgidum ssp. turgidum landrace carrying dwarf gene in China. Its dwarfing trait was found to be sensitive to gibberellic acid. In 2012, we crossed Ailanmai with two high plant landraces, Qinkemai and Ganmai, and obtained their reciprocal F1 hybrids. The genetic analysis was carried out in Mianyang, Sichuan Province using the F1, F2, and F2:3 populations during the 2012–2013 crop seasons. One recessive gene was proved to control the dwarfing trait in Ailanmai. Poly-morphic simple sequence repeat (SSR) primers associated with plant height were selected through bulked segregant analysis (BSA) and used to identify the F2 individuals. The results indicated that the dwarf gene was located on the short arm of chromosome 7A with a genetic distance of 2.5 cM from marker GWM471. We speculated Rht22 to be the dwarf gene in Ailanmai because the re-ciprocal F1 and F2 hybrids between Ailanmai and Aiganfanmai (carrying Rht22) exhibited similar distributions in plant height. This speculation was validated with high-through molecular marker analysis. The percentages of identical SNP and DArT markers between Ailanmai and Aiganfanmai were as high as 98.7% and 99.3%, respectively. We conclude that the two landraces might be the same variety a long time ago and became synonymic during their spread accompanying with humanity activities. The dwarf gene in Ailanmai had a moderate or weak effect to reduce plant height in synthetic hexaploid wheat. Thus, it should be utilized by pyramiding other dwarfing genes in wheat dwarfing breeding.关键词
矮蓝麦/赤霉酸敏感型/遗传分析/Rht22/圆锥小麦Key words
Ailanmai/Gibberellic acid sensitive/Genetic analysis/Rht22/Triticum turgidum ssp. turgidum引用本文复制引用
周强,袁中伟,张连全,甯顺腙,任勇,陶军,李生荣,刘登才..四倍体小麦地方品种矮蓝麦矮秆性状的遗传分析[J].作物学报,2015,(12):1899-1905,7.基金项目
本研究由国家现代农业产业技术体系建设专项(CARS-3-2-40),四川省“十二五”育种攻关资助项目(2011YZGG-3)和国家现代农业产业技术体系四川省麦类创新团队项目资助。This study was supported by the grants from the Modern Agro-industry Technology System (CARS-3-2-40), and the 12th Five-year Breeding Re-search Project in Sichuan Province (2011YZGG-3), and the Modern Agro-industry Technology System of Sichuan Triticeae Innovation Team （CARS-3-2-40）
