作物学报2016，Vol.42Issue(1)：1-10,10.DOI:10.3724/SP.J.1006.2016.00001
GmMYB042基因对类黄酮生物合成的调控作用
Regulating Effects of GmMYB042 Gene on Flavonoid Biosynthesis
摘要
Abstract
MYB transcription factor is one of the largest transcription factor gene families in land plants, and is involved in a myriad of regulatory processes, such as secondary metabolism. In the present study, the expression profiles and function of GmMYB042 gene were systematically studied. In order to investigate the roles of the conserved amino acid motif PDLNLELTIS and a predicted zinc finger region at its C-terminal, a series of sequence deletions of these two regions were made by PCR method. Subsequently, the corresponding over-expression constructs of GmMYB042 gene and its mutants were made and transformed into tobacco NC89 with Agrobacterium LBA4404, respectively. Expression analyses revealed that GmMYB042 gene was expressed in nodule, root, stem, leaf, flower, pod, and seed of soybean, and with a relative higher expression level in stem, flower, and seed;its expression could be induced by PEG, high salt, low temperature, and UV-B radiation stresses. Over-expression analyses showed that the expressions of some enzyme genes in flavonoid biosynthesis pathway (including PAL, CHS, CHI, and FLS) were obviously increased in GmMYB042 transgenic lines, resulting in an increased content of the flavonoid compounds. Accordingly, the transcription levels of the corresponding enzyme genes involved in flavonoid biosynthesis pathway were decreased in the transgenic lines of GmMYB042 mutants, further supporting the conclusion of regulating role of GmMYB042 gene in tobacco fla-vonoid biosynthesis pathway.关键词
MYB转录因子/类黄酮/缺失突变/功能研究Key words
MYB transcription factor/Flavonoid/Deletion mutation/Functional research引用本文复制引用
杜海,冉凤,马珊珊,柯蕴倬,孙丽萍,李加纳,唐益雄..GmMYB042基因对类黄酮生物合成的调控作用[J].作物学报,2016,42(1):1-10,10.基金项目
本研究由国家自然科学基金项目(31471528),国家博士后基金面上项目(2014M552297)和中央高校基本科研业务费专项资金(SWU113104, XDJK2014B035,2362015xk05)资助。This study was supported by National Natural Science Foundation of China (31471528), Postdoctoral Science Foundation of China (2014M552297) and Fundamental Research Funds for the Central Universities of China (SWU113104, XDJK2014B035,2362015xk05) （31471528）
