作物学报2016，Vol.42Issue(1)：11-18,8.DOI:10.3724/SP.J.1006.2016.00011
小麦果聚糖合成酶基因6-SFT-D多态性及其与6-SFT-A2的累加效应
Sequence Polymorphism and Cumulative Effect with 6-SFT-A2 of Fructan Bio-synthesis Gene 6-SFT-D in Wheat
摘要
Abstract
Gene 6-SFT encodes a key enzyme in fructan biosynthesis pathway in common wheat (Triticum aestivum L.). In this study, we analyzed the single nucleotide polymorphism (SNP) on 6-SFT-D locus in a diversity population of 23 hexaploid wheat (AABBDD) accessions and five wheat relative species (DD) by means of direct sequencing. Functional markers were developed according to the sequence polymorphism. The correlation between 6-SFT-D haplotypes and phenotypic traits and the cumulative effect of 6-SFT alleles were analyzed using a natural population consisting of 154 historical wheat accessions. Four SNPs were detected in 6-SFT-D sequences in the diversity population, forming three haplotypes. However, only two 6-SFT-D haplotypes were identified in the natural population. We developed a pair of allele-specific PCR markers based on a polymorphism (T/C) at the 2850 bp site. The results of haplotype–trait association analysis showed that 6-SFT-D was significantly associated with thou-sand-grain weight (TGW) and spike length under well-watered conditions. Hap I was a superior allele in improving TGW. Under drought stress and well-watered conditions, wheat materials carrying both superior allele of 6-SFT-D and 6-SFT-A2 had signifi-cantly higher TGW than other genotypes, suggesting that 6-SFT-D and 6-SFT-A2 have cumulative effect on TGW improvement.关键词
普通小麦/6-SFT-D/功能标记/单核苷酸多态性/关联分析/累加效应Key words
Wheat/6-SFT-D/Functional marker/SNP/Association analysis/Cumulative effect引用本文复制引用
岳爱琴,李昂,毛新国,昌小平,柳玉平,李润植,景蕊莲..小麦果聚糖合成酶基因6-SFT-D多态性及其与6-SFT-A2的累加效应[J].作物学报,2016,42(1):11-18,8.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2011AA100501)和国家自然科学基金项目(31461143024)资助。This study was supported by the National High Technology Research and Development Program of China (863 Program)(2011AA100501) and the National Natural Science Foundation of China (31461143024) （863计划）
