作物学报2016，Vol.42Issue(1)：31-42,12.DOI:10.3724/SP.J.1006.2016.00031
聚合稻瘟病、白叶枯病和褐飞虱抗性基因的三系恢复系改良效果的评价
Evaluation of Improvement Effect of Restorer Lines on Pyramiding Genes Re-sistant to Rice Blast, Bacterial Leaf Blight and Brown Planthopper
摘要
Abstract
The novel blast resistance genes, Pi-GD-1(t) and Pi-GD-2(t) (abbreviated as G1 and G2, respectively) from rice culti-var Sanhuangzhan2, bacterial blight resistance gene Xa23 (abbreviated as X) from CBB23 and brown planthopper resistance gene Bph18(t) (abbreviated as B) from IR65482-7-216-1-2-B (abbreviated as IR65482) were introgressed into three rice restorer lines, Wenhui 845, Wenhui 117 and Wenhui 143, by marker-assisted backcross and pyramiding breeding methods integrated with artifi-cial inoculation, natural induction and phenotypic selections. The eight resistant restorer lines pyramiding blast and brown planthopper resistance genes, i.e. Wenhui 845-G1-G2-B-4, Wenhui 845-G1-G2-B-5, Wenhui 117-G1-G2-X-B-3, Wenhui 143-G1-G2-B-3, Wenhui 143-G2-X-B-9, Wenhui 143-G2-X-B-10, Wenhui 143-G1-G2-B-11, and Wenhui 143-G1-G2-B-37, and their tester lines crossed with the sterile line Wufeng A demonstrated the similar or slight lower level of resistance against rice blast and brown planthopper as compared with the resistant donor parents Sanhuangzhan 2 or IR65482. Part of developed restorer lines (Wenhui 117-G1-G2-X-B-3, Wenhui 143-G2-X-B-9, and Wenhui 143-G2-X-B-10) and their tester lines showed resistance or moderate resistance to bacterial blight. The newly developed restorers resistant to blast, bacterial blight and brown planthopper were similar or superior to their respective original types in agronomic traits under normal condition, implying these resistant restorer lines can be useful in hybrid rice breeding and production. The results indicated that Xa23 could completely express its dominant resistance against different restorer genetic backgrounds whereas effects of resistance improvement in Pi-GD-1(t), Pi-GD-2(t), and Bph18(t) depend on their genetic background.关键词
水稻恢复系/稻瘟病/白叶枯病/褐飞虱/抗性改良/标记辅助选择Key words
Rice restorer lines/Blast/Bacterial blight/Brown planthopper/Resistance improvement/Marker-assisted selection引用本文复制引用
楼珏,杨文清,李仲惺,罗天宽,谢永楚,郑国楚,岳高红,徐建龙,卢华金..聚合稻瘟病、白叶枯病和褐飞虱抗性基因的三系恢复系改良效果的评价[J].作物学报,2016,42(1):31-42,12.基金项目
本研究由浙江省农业科研重大专项(“8812”计划,2012C12901-17),温州市种子种苗科技创新专项(N20120019),浙江省教育科学规划课题(2015SCG183)和浙江省教育厅一般科研项目(Y201533916)资助。This study was supported by Special Projects of the Zhejiang Agricultural Key Research (8812 Plan,2012C12901-17) and Wenzhou Seeds and Seedlings Technology Innovation (N20120019), Project of the Zhejiang Education Science and Planning (2015SCG183) and General Research Program for the Education Department of Zhejiang Province (Y201533916) （“8812”计划,2012C12901-17）
