作物学报2016，Vol.42Issue(1)：51-57,7.DOI:10.3724/SP.J.1006.2016.00051
DTA-6对大豆花荚脱落纤维素酶和GmAC基因表达的调控
Regulation of DTA-6 by Abscission Cellulase and GmAC Gene Expression in Flowers and Pods of Soybean
摘要
Abstract
It is effective to regulate abscission of soybean flowers and pods by spraying plant growth regulators (PGRs). This study was carried out to determine the effect of DTA-6 on abscission cellulase (AC) activity, abscission cellulase gene (GmAC) expressive, abscission of flowers and pods, and yield in soybean. DTA-6 was foliage sprayed at R1 stage on three varieties of Suinong 28, Kenfeng 16, and Hefeng 50 in 2012 and 2013. The results demonstrated that DTA-6 treatment inhibited GmAC gene expression in abscission zone of flower and pod, with the maximum reduction of 51%among three varieties compared with the control. The abscission cellulase activity was periodically decreased by DTA-6, with different decrements among soybean culti-vars. DTA-6 significantly decreased (P<0.05) abscission rate of soybean flowers and pods, and significantly increased yield (P<0.05). The relative expression of abscission cellulase gene (GmAC) in abscission zone of flowers-pods was decreased and the AC activity was regulated by DTA-6, resulting in reduced abscission rate of soybean flowers and pods and promoted yield.关键词
DTA-6/GmAC基因表达/脱落纤维素酶/花荚脱落率/产量Key words
DTA-6/GmAC expression/Abscission cellulase/Flowers and pods abscission/Yield引用本文复制引用
崔洪秋,冯乃杰,孙福东,刘涛,李建英,杜吉到,韩毅强,郑殿峰..DTA-6对大豆花荚脱落纤维素酶和GmAC基因表达的调控[J].作物学报,2016,42(1):51-57,7.基金项目
本研究由国家自然科学基金项目(31171503,31271652),国家科技支撑计划项目(2012BAD20B04),黑龙江省研究生创新科研项目(YJSCX2012-253HLJ),黑龙江省杰出青年基金项目(JC201309)和黑龙江农垦总局科技攻关项目(HNK12A-06-03, HNK12A-09-02)资助。This study was supported by the National Natural Science Foundation of China (31171503,31271652), the National Key Technology R&D Program of China (2012BAD20B04), Heilongjiang Provincial Graduate Science and Technology Innovation Projects for Graduate Sdutents (YJSCX2012-253HLJ), Heilongjiang Provincial Funds for Distinguished Young Scientists (JC201309), and Key Scientific and Technological Projects from Heilongjiang Agriculture and Reclamation Bureau (HNK12A-06-03, HNK12A-09-02) （31171503,31271652）
