中医药学报Issue(5)：70-76,7.
基于LC／QTOF－MS／MS法鉴定兰考泡桐花化学成分
Structural Identification of Compounds in Paulownia elongata Flower by Liquid Chromatography Coupled with Electrospray Ionization Quadrupole Time-of-flight Tandem Mass Spectrometry
摘要
Abstract
Objective:To identify the compounds in Paulownia elongata flower preliminarily and provide data to support the study on the effective material basis of this herb .Methods:Liquid chromatography coupled with electrospray ioniza-tion quadrupole time-of-flight tandem mass spectrometry ( LC/ESI-QTOF-MS/MS) was performed in the positive mode to analyze the compounds in the extracts of Paulownia elongata flower .The mobile phase consisted of water with 0.1% formic acid ( A) and acetonitrile ( B) using a gradient elution .Results:12 compounds (11 flavonoids and 1 ur-solic acid) were selectively identified in Paulownia elongata flower .Five compounds were unambiguously identified by comparing with the reference compounds and 7 compounds were tentatively identified or deduced according to their MS /MS data .Conclusion:Logical fragmentation pathways for flavonoids and ursolic acid have been proposed and are useful for the identification of these types of compounds in paulownia plants especially in the case of no chemical reference com -pounds .关键词
兰考泡桐花/LC/ESI-QTOF-MS/MS/黄酮/熊果酸/化学表征Key words
Paulownia elongata flower/LC/ESI-QTOF-MS/MS/Flavonoids/Ursolic acid/Chemical properties分类
医药卫生引用本文复制引用
王清,刘敬,陈晓兰,张建,刘丽芳,辛贵忠..基于LC／QTOF－MS／MS法鉴定兰考泡桐花化学成分[J].中医药学报,2015,(5):70-76,7.基金项目
国家自然科学基金资助项目（30960495）；江苏高校优势学科建设工程资助项目 （）
