北京林业大学学报2015，Vol.37Issue(11)：69-75,7.DOI:10.13332/j.1000-1522.20140485
白桦BpHO基因非生物胁迫及信号诱导的表达模式分析
Analysis of the expression patterns of BpHO gene induced by abiotic stress and signal in birch
摘要
Abstract
Heme oxygenase ( HO ) is the rate-limiting enzyme in the process of heme catabolism which is ubiquitous, sensitive, and highly active . This paper reveals the BpHO expression pattern in signal induction and regulation of secondary metabolism, in an attempt to lay the foundation for the study of gene function in metabolic regulation of birch. In our previous research, the HO gene has been cloned from birch ( Betula platyphylla ) , named BpHO. The molecular structure of BpHO was analyzed by bioinformatics software. Abiotic stress by cadmium ( Cd) , salt ( NaCl) and low temperature (4℃) , and signal induced by sodium nitroprusside ( SNP) and salicylic acid ( SA) were used in the study. The expression patterns of BpHO induced by abiotic and signal induction were analyzed. Bioinformatics analysis showed that BpHO is 855 bp, and contains the complete ORF, encoding 284 amino acids ( Genebank ID:KJ197335 ) . BpHO is an unstable hydrophobic protein, which does not possess signal peptide and the transmembrane ability either. The alpha helix, extension chain and random coil are distributed throughout the protein. Molecular evolution analysis results showed that the genetic distance of BpHO gene between birch and grape species is relatively close. The gene heredity distance is farther with soybean, Kidney bean and pea, explaining the far genetic relationship with them. Abiotic stress results indicated that BpHO gene expression levels varied with abiotic stress time with fluctuations, but after 6 h treatment BpHO gene for four abiotic stress treatments were up-regulated, indicating BpHO genes are responsive to abiotic stresses. The results suggest that the signal induced by exogenous NO regulates transcription expression of BpHO and the synthesis of heme oxygenase. Salicylic acid is also involved in expression of BpHO. Salt, low temperature, heavy metal, and cadmium stress can promote the expression of BpHO, while the response patterns differ.关键词
白桦/BpHO/生物信息学分析/非生物胁迫Key words
Betula platyphylla/BpHO/bioinformatics analysis/abiotic stress分类
农业科技引用本文复制引用
周姗,李思达,董恒,詹亚光,曾凡锁..白桦BpHO基因非生物胁迫及信号诱导的表达模式分析[J].北京林业大学学报,2015,37(11):69-75,7.基金项目
中央高校基本科研业务费专项资金项目(2572014DA04)、国家自然科学基金项目(J1210053、31200463)、黑龙江省博士后启动基金项目(LBH-Q12166). （2572014DA04）
