动物医学进展Issue(12)：1-5,5.
鸭疫里默菌aroA基因缺失突变株的构建及生物学特性研究
Construction and Characterization ofR.anatipestifer aroA Deletion Strain
摘要
Abstract
R .anatipestifer aro A deletion strain was constructed and its biological characteristics and viru-lence were detected.Suicide recombinant plasmid of pRE112-LSR was constructed,and the aro A gene in R .anatipestifer YM strains was successfully deleted by conjugation and identified by PCR.The results showed that the main biological characteristics were similar with the wild-type strain,while the aro A mu-tant could not hydrolyze arginine;Growth rate of the aro A mutant in vitro was slower than wild-type strain within logarithmic phase,and similar to the wild-type strain at stable phase;LD50 of the aro A mu-tant was decreased three times than that of wild-type strain;Quantity of isolated bacteria from ducking tis-sues showed that the aro A mutant was less than it in blood,heart,liver and brain of wild-type strain,but it was not significant.These results provided a good foundation for the further study of the molecular path-ogenesis of R .anatipestifer and development of genetic engineering vaccines.关键词
鸭疫里默菌/aro A 基因/基因缺失/生物学特性/毒力因子Key words
R .anatipestifer/aro A gene/gene deletion/biological characteristic/virulence factor分类
农业科技引用本文复制引用
张硕,李自力,刘宁,卢媞,王颖,周祖涛,刘梅,胡思顺,栗绍文,毕丁仁..鸭疫里默菌aroA基因缺失突变株的构建及生物学特性研究[J].动物医学进展,2015,(12):1-5,5.基金项目
武汉市科技计划项目 （）
