动物医学进展Issue(12)：56-61,6.
小鼠和奶牛乳腺上皮细胞的分离及培养特性比较
Comparison the Isolation and Culture Characteristics Between Mouse Mammary Epithelial Cells and Bovine Mammary Epithelial Cells
摘要
Abstract
The objective of the study was the comparison of culture characteristics between mouse mammary epithelial cells (MMECs)and bovine mammary epithelial cells (BMECs),and establishing the isolation systems.The breast tissues from late pregnancy KM mice and mid-lactation Holstein cows were collected aseptically,then modified enzyme mixture combined with differential adherence time was used to digest and separate primary MMECs and BMECs.Finally,the MMECs and BMECs were identified via cytokera-tin18 expression by immunofluorescence staining.The biological characteristics of MMECs and BMECs were evaluated by cell morphology observation after cell passage,cryopreservation and resuscitation. Growth curves of MMECs and BMECs were made by cell culture and counting continuously.The results showed that those cells separated from breast tissues were MMECs and BMECs;The confluent monolayer MMECs were polygonal and paving stone appearance, while BMECs were cobblestone appearance;MMECs’specific morphology disappeared when passage 2nd generation,in contrast to MMECs,BMECs were holding cobblestone appearance until the passage 9th generation;Compared to MMECs who can only be cryopreservated and resuscitated once,each passage BMECs can be cryopreservated and resuscitated.In conclusion,there are significant differences in the growth and culture characteristics of these isolated high-purity MMECs and BMECs.关键词
小鼠乳腺上皮细胞/奶牛乳腺上皮细胞/分离/培养/生长特性Key words
mouse mammary epithelial cells/bovine mammary epithelial cell/isolation/culture/growth characteristic分类
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刘玉辉,赖金伦,寇明明,邓明亮,刘瑞宁,陈颖钰,胡长敏,郭爱珍..小鼠和奶牛乳腺上皮细胞的分离及培养特性比较[J].动物医学进展,2015,(12):56-61,6.基金项目
国家肉牛／牦牛产业技术体系（nycytx-38）；宁夏肉牛呼吸疾病综合征及皮肤病防控技术研究与示范（2012ZDN0903）；中央高校基本科研业务费专项资金资助项目（2662015PY054）；湖北省自然科学基金项目 （）
