福建农林大学学报（自然科学版）2015，Vol.44Issue(6)：639-645,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2015.06.014
不同微量元素对斑玉蕈菌丝生长及酶活的影响
Effects of trace elements on the mycelial growth and enzyme activities of Hypsizygus marmoreus
摘要
Abstract
Effects of trace elements on the mycelial growth rate and activities of some enzymes related to substrate degradation of Hypsizigus marmoreus were explored under different cultivation conditions by addition of six trace elements in enriched PDA media and production media. The results showed some metal ions ( Mn2+, Ca2+, Mo6+and Fe2+) could significantly accelerate the mycelial growth in enriched PDA media with the concentration of 100, 60, 500 and 100 mg?L-1 , respectively. Adding Fe2+and Cu2+in pro-duction media had remarkable promoting effects on mycelial growth. Zinc ion inhibited the growth of mycelia when it was added to production media. Copper ion supplementation ( 100 mg?kg-1 ) in production media was most beneficial to the activities of laccase, manganese peroxidase and lignin peroxidase ( reaching 39.83, 19.17 and 9.58 U?mL-1 , respectively) . However, there were no sig-nificant effects of all trace elements on the activities of cellulase and xylanase. It suggested that different trace elements had different effects on the mycelial growth of H.marmoreus and the activities of its related enzyme in different substrates.关键词
斑玉蕈/微量元素/菌丝生长速度/酶活Key words
Hypsizygus marmoreus/trace element/mycelial growth rate/enzyme activity分类
农业科技引用本文复制引用
孙淑静,单书凯,李钊锋,郭艳艳,陈文星,胡开辉..不同微量元素对斑玉蕈菌丝生长及酶活的影响[J].福建农林大学学报（自然科学版）,2015,44(6):639-645,7.基金项目
福建省发改委农业五新工程项目(闽发改委投资[2012]931号) （闽发改委投资[2012]931号）
福建省自然基金杰出青年项目(2014J06010) （2014J06010）
福建农林大学校杰出青年人才项目(xjq201209) （xjq201209）
国家自然基金青年项目(31201669) （31201669）
教育部博士点基金资助项目(20103515120015). （20103515120015）
