军事医学Issue(11)：868-872,5.DOI:10.7644/j.issn.1674-9960.2015.11.014
环介导恒温扩增技术快速检测鼠疫耶尔森菌
Loop-mediated isothermal amplification for quick detection of Yersinia pestis
摘要
Abstract
Objective To establish a simple and quick loop-mediated isothermal amplification (LAMP)method for detection of Yersinia pestis.Methods LAMP Primers were designed based on the specific sequence 3a in Y.pestis chromosome.LAMP reaction results were detected using turbidity meter or visual method.The specificity of the constructed method was evaluated by detecting Y.pestis and its closely-related bacteria.The different dilution DNA template was detected with LAMP and PCR to evaluate the sensitivity of the method.Results Thirty strains of bacteria closely related to Y.pestis were detected by the constructed LAMP,and all the results were negative,indicating that the method had a very high specificity.The detection sensitivity of this LAMP assay was 20 pg of DNA per reaction,which was ten-fold that of the regular PCR.The detection reaction was completed in 25 min.Conclusion This LAMP method is quick,sensitive, specific and simple,which is expecked to become an effective method for rapid detection of Y.pestis on the scene.关键词
鼠疫耶尔森菌/环介导恒温扩增技术/检测Key words
Yersinia pestis/loop-mediated isothermal amplification/detection分类
医药卫生引用本文复制引用
冯娜,周亚洲,范艳晓,汪琼,毕玉晶,韩延平,杨瑞馥,周育森,王效义..环介导恒温扩增技术快速检测鼠疫耶尔森菌[J].军事医学,2015,(11):868-872,5.基金项目
国家自然科学基金资助项目（31430006，81171529）；国家重点基础研究发展计划课题 （）
