水生态学杂志Issue(5)：63-68,6.DOI:10.15928/j.1674-3075.2015.05.010
锦鲤源铜绿假单胞菌的分离鉴定及药敏试验
Isolation,Identification and Drug Sensitivity Test of Pathogenic Pseudomonas aeruginosa from Koi
摘要
Abstract
To identify the pathogen that caused serious mortality of koi cultured in Jiangsu Province during August 2014,the pathogenic strain was isolated from diseased fish and labeled the HL2 strain.After purification,the iso-lated strain was cultured by incubation in LB medium.Healthy koi with body length 7 -10 cm and body mass (25 ±3)g were infected with strain HL2.Sixty healthy koi were randomly assigned into three control groups and three test groups,with 10 koi in each group.The koi in the test groups were injected with 0.2 mL bacterium suspension at a concentration of 1 ×108 CFU /mL and the control groups were injected with an equal volume of LB medium. The mortality rate of each group was recorded daily and the pathogenic strain from dead koi was isolated to confirm the cause of death was infection by strain HL2.The isolated bacteria strain HL2 was highly pathogenic and healthy koi in the test groups were all dead within 14 days.Clinical symptoms in artificially infected koi were similar to those observed in koi naturally infected.Physiological and biochemical characteristics of the isolated HL2 strain were detected by Gram-stain and 16S rRNA gene analysis.Bacterial strain HL2 is gram-negative rod shape and mo-tile and the length of the 16S rRNA gene sequence was 1 461 bp (Genbank login number:KF413420).Phyloge-netic tree analysis shows that,based on the 16S rRNA sequence,HL2 clusters with and is a strain of Pseudomonas aeruginosa.Drug susceptibility of strain HL2 was tested by the disc agar diffusion (K-B)method using 55 test anti-biotics:35 antibiotics,including Ceftriaxone,Kanamycin and Tetracycline showed obvious inhibition;5 antibiot-ics,including Gentamicin,Doxycycline and Cefradine showed moderate inhibition;15 antibiotics including Penicil-lin G,Ampicillin and Cefazolin showed no inhibition.Our study provides information for preventing and treating strain HL2 infection in koi and basic data for further research on Pseudomonas aeruginosa.关键词
锦鲤/铜绿假单胞菌/分离鉴定/药敏特性Key words
koi/Pseudomonas aeruginosa/isolation and identification/antibiotic sensitivity分类
海洋科学引用本文复制引用
陈凯,梁利国,谢骏..锦鲤源铜绿假单胞菌的分离鉴定及药敏试验[J].水生态学杂志,2015,(5):63-68,6.基金项目
江苏省水产三新工程（D2013-5；D2015-13）；现代农业产业技术体系建设专项（CARS-46-10）。 （）
