水生态学杂志Issue(5)：96-100,5.DOI:10.15928/j.1674-3075.2015.05.015
盐度和光照对泥蚶耗氧率和排氨率的影响
Effects of Salinity and Light on Oxygen Consumption Rate and Ammonia Excretion Rate of Tegillarca granosa
摘要
Abstract
Tegillarca granosa,also called the blood clam,is an important species in traditional shellfish farming, high in nutrient content and economic value.Respiration and excretion rates are typically used to indicate their lev-el of physiological activity.To better understand the metabolic intensity and circadian rhythms of T.granosa under different environmental conditions,we investigated the effects of salinity and light on the physiological activity of T. granosa and the results provide a theoretical basis for scientific culture.Physiological indices of respiration and ex-cretion were expressed by oxygen consumption rate and ammonia excretion rate.In August,2014,healthy blood clams were randomly assigned to five groups,acclimated to and then tested in duplicate at five salinities(20,24, 28,30 and 32 g/kg)and a temperature of 25℃ with static cultivation.After acclimation,five clams were placed in 2.5 L bottle respirators with water of the salinity being tested and the bottle respirators were sealed when the blood clams opened their shells.Dissolved oxygen and ammonia nitrogen were measured with a CM-07 aquaculture water quality analyzer before the test began and again after 2 h and the initial and final concentrations were used to calculate the rate of oxygen consumption and rate of ammonia excretion.Results show that that the oxygen consump-tion rate and ammonia excretion rate of T.granosa both increased initially and then decreased as salinity increased. The oxygen consumption rate was in the range of 0.33 -0.48 mg/(g·h),and the ammonia excretion rate was in the range of 0.044 -0.067 mg/(g · h).The low and high values of both rates were significantly different (P <0.05)and both rates were highest at a salinity of 28 g/kg group.While T.granosa tolerated all five of the salinities tested,the optimal salinity for T.granosa is 28 g/kg.The respiration and excretion rate of T.granosa was also studied under light and dark conditions.Blood clams acclimated to a salinity of 28 g/kg were used and five clams were again placed in each respirator and light and dark treatments were run in triplicate.Otherwise,the test method was the same as the salinity test.Under dark conditions,the oxygen consumption rate ranged from 0.42 mg/(g·h)to 0.53 mg/(g · h)and the ammonia excretion rate ranged from 0.049 mg/(g · h)to 0.057 mg/(g·h).Under light conditions,the oxygen consumption rate ranged from 0.40 mg/(g · h)to 0.44 mg/(g·h)and the ammonia excretion rate ranged from 0.042 mg/(g·h)to 0.051 mg/(g·h).T. granosa displayed higher rates of respiration and excretion in the dark but the difference was not significant (P >0.05).Therefore,we can conclude that the growth of T.granosa has no obvious circadian rhythm.关键词
泥蚶/盐度/光照/耗氧率/排氨率Key words
Tegillarca granosa/salinity/light/oxygen consumption rate/ammonia excretion rate分类
生物科学引用本文复制引用
熊安安,袁星,汪先进,林,黄建荣,黎祖福..盐度和光照对泥蚶耗氧率和排氨率的影响[J].水生态学杂志,2015,(5):96-100,5.基金项目
海域使用金项目－广东海岸带生态修复及保护示范及建设（201305021）；国家海洋公益性行业科研专项－典型滨海湿地修复效果评估与功能保育（2013418021-5）。 （）
