生物加工过程Issue(6)：6-12,7.DOI:10.3969/j.issn.1672-3678.2015.06.002
酿酒酵母产D 乳酸重组菌的构建与发酵
Construction of metabolically engineered Saccharomyces cerevisiae for D-lactic acid production
摘要
Abstract
A Saccharomyces cerevise strain was constructed by genetic engineering method,with lactic acid metabolic pathways. The gene dldh,encoding a lactate dehydrogenase from L. mesenteroides and E. coli, was cloned into a yeast shuttle vector PYX212⁃kanMX. The resultant plasmid pYX212⁃kanMX⁃DLDH was introduced into W303⁃1A by electroporation method and obtained two recombinant S. cerevisiae WE1510 and S. cerevisiae WB1186. Subsequently, a recombinant D⁃lactic acid producing yeast WB1186 was obtained,which had a better ability to produce D⁃lactic acid. It was up to 18�0 g/L of the lactic acid accumulation by batch fermentation in a 3⁃L fermenter for 96 h and the preliminary fermentation conditions were as follows, culture medium YEPD, inoculating rate 10%, DO 30%, stirring rate 150 r/min,concentration of glucose 10 g/L,pH 5�0, aeration rate 3 L/min,when OD600 up to maximum, the fermentation was turned to anaerobic.关键词
D 乳酸/D 乳酸脱氢酶/Leuconostoc mesenteroides/酿酒酵母/代谢改造Key words
D-lactic acid/DLDH/Leuconostoc mesenteroides/Saccharomyces cerevisiae/metabolically engineered分类
化学化工引用本文复制引用
汪兆峰,石贵阳,张梁..酿酒酵母产D 乳酸重组菌的构建与发酵[J].生物加工过程,2015,(6):6-12,7.基金项目
国家高技术研究发展计划（863计划）（2011AA02A205、2012AA021201）；教育部新世纪优秀人才支持计划 （）
