生物加工过程Issue(6)：13-17,5.DOI:10.3969/j.issn.1672-3678.2015.06.003
碱预处理玉米芯米根霉同步糖化发酵产富马酸
Simultaneous saccharification and fermentation of alkali-pretreated corncob to fumaric acid by Rhizopus oryzae
摘要
Abstract
Simultaneous saccharification and fermentation ( SSF) of alkali⁃pretreated corncob to fumaric acid by Rhizopus oryzae was optimized. The optimal conditions were as follow:substrate loading 50 g/L, ammonium sulfate 0�71 g/L,cellulase dosage 20 FPIU/g cellulose,calcium carbonate 30 g/L,inoculum 10%(V/V),and loading volume 50 mL. Simultaneous saccharification and fermentation(SSF) of alkali⁃pretreated corncob to fumaric acid was compared to direct fermentation of glucose. Under the optimal conditions, process of SSF by Rhizopus oryzae could produce 13�78 g/L fumaric acid, while direct fermentation only obtained 6�21 g/L fumaric acid using glucose as the carbon source.关键词
碱预处理/玉米芯渣/富马酸/同步糖化发酵/米根霉Key words
alkali-pretreated/corncob/fumaric acid/simultaneous saccharification and fermentation/Rhizopus oryzae分类
化学化工引用本文复制引用
顾夕梅,周瑾,陈云,李鑫..碱预处理玉米芯米根霉同步糖化发酵产富马酸[J].生物加工过程,2015,(6):13-17,5.基金项目
国家科技支撑计划（2015BAD15B09）；江苏省高校自然科学研究重大项目（14KJA220003）；江苏省自然科学基金 （）
