生物加工过程Issue(6)：18-23,6.DOI:10.3969/j.issn.1672-3678.2015.06.004
大孔树脂共吸附固定葡聚糖脂肪酶催化合成香茅酯
Co-immobilization of dextran and Candida rugosa lipase on macroporous resin and synthesis of citronellyl esters
摘要
Abstract
Lipase and dextran were co⁃immobilized on a macroporous resin synchronously,the influence on co⁃immobilization of dextran was investigated and the immobilized lipase was used as the catalyst for the synthesis of citronellyl laurate. As a result,the co⁃immobilization of dextran with final concentration of 0�75 mg/mL during the immobilization procedure improved enzyme activity recovery and thermal stability,the esterification rate reached 95�3%after 10 h,when 1 U of immobilized lipase was dosed and the reaction was carried out at 50℃ in the solvent⁃free system with the molar ratio of lauric acid to citronellol of 1∶1. More importantly,the reaction time could be further shortened to 6 h by adding dextran T⁃20 (Mw=2×104) and T⁃40 (Mw=4×104) with final concentration of 0�75 mg/mL during the immobilization procedure,and the relative activity of immobilized lipase was still above 75% after ten recycles.关键词
脂肪酶/葡聚糖/共固定/无溶剂/月桂酸香茅酯Key words
lipase/dextran/co-immobilization/solvent-free/citronellyl laurate分类
生物科学引用本文复制引用
于敏,周海燕,任立伟,蒋振华,周华,韦萍..大孔树脂共吸附固定葡聚糖脂肪酶催化合成香茅酯[J].生物加工过程,2015,(6):18-23,6.基金项目
国家重点基础研究发展计划（973计划） （）
