生物加工过程Issue(6)：75-80,6.DOI:10.3969/j.issn.1672-3678.2015.06.014
纤维素酶法提取川牛膝多糖
Cellulase-assisted extraction of polysaccharide from Cyathula officinalis Kuan
摘要
Abstract
Cellulase was applied to extract polysaccharide from Cyathula officinalis Kuan. Factors influencing particle size of the herb, the concentration of cellulase, enzyme treatment temperature and time, solvent pH value, liquid to solid ratio and extractive duration were studied. The optimal conditions were verified by the orthogonal experiment. The optimum extracting parameters were as follows:particle size 550 to 830 μm,enzyme concentration 4 mg/g, temperature 50 ℃,enzyme treatment time 90 min, pH=5�0,the liquid to solid ratio 60, extractive duration 30 min. Under these conditions, the yield of polysaccharide from Cyathula officinalis Kuan was 71�70%.关键词
川牛膝/多糖/纤维素酶/提取Key words
Cyathula officinalis Kuan/polysaccharide/cellulase/extraction分类
医药卫生引用本文复制引用
谷旭晗,刘傲霞,成悦,夏玉婷,韩伟..纤维素酶法提取川牛膝多糖[J].生物加工过程,2015,(6):75-80,6.基金项目
国家大学生创新创业训练计划 （）
