中国医药科学Issue(19)：61-64,111,5.
Plackett-Burman试验、最速上升试验及Box-Behnken试验联用优化栀子多酚超声提取工艺
Optimization of ultrasonic extraction technology of polyphenols fromGardenia jasminoides Ellis by Plackett-Burman,the steepest ascent and Box-Behnken experiment
摘要
Abstract
ObjectiveTo optimize the ultrasonic extraction process of polyphenols fromGardenia jasminoides Ellis.Methods The significant factors were selected from the influence factors of concentration of ethanol, particle diameter、the ratio of liquid to material, ultrasonic power, ultrasonic temperature, ultrasonic time and extraction times by Placket-Burman experiment, then the central point of response surface was determined by the Steepest ascent experiment, finally the ultrasonic extraction process was optimized by Box-Behnken experiment.ResultsOptimum ultrasonic extraction process was as follows: concentration of ethanol 45%, the ratio of liquid to material 25:1, ultrasonic time 35min, the total yield of polyphenol was 1.857%, close to the predicted value of 1.9%.Conclusion The optimized extraction technology which provided references for the exploitation and utilization ofGardenia jasminoides Ellis was scientific and reasonable.关键词
栀子/多酚/Plackett-Burman试验/最速上升试验/Box-Behnken试验Key words
Gardenia jasminoidesEllis/Polyphenol/Plackett-Burman experiment/Steepest ascent experiment/Box-Behnken experiment分类
医药卫生引用本文复制引用
黄潇,肖雄,高文军,陈瑶..Plackett-Burman试验、最速上升试验及Box-Behnken试验联用优化栀子多酚超声提取工艺[J].中国医药科学,2015,(19):61-64,111,5.基金项目
江西中医药大学科研基金（2013jzzdxk046）。 （）
