中国免疫学杂志2015，Vol.31Issue(12)：1591-1594,4.DOI:10.3969/j.issn.1000-484X.2015.12.002
TNFSF14及其受体LTβR和HVEM在病毒肝炎中的作用
Role of TNFSF14 and its receptors LTβR and HVEM in pathogenesis of virus hepatitis
摘要
Abstract
Objective:To explore the role of TNFSF14 and its receptors LTβR and HVEM in the pathogenesis of virus hepatitis.Methods:Marine fulminant viral hepatitis model was established by infecting mice with MHV-3.Liver tissue destruction in LIGHT KO and WT mice were analyzed by HE staining and ALT levels in serum by automatic biochemical analyzer .The mRNA levels of HVEM and LTβR in the liver and spleen tissues in the indicated time points ( 0 h, 12 h, 24 h, 48 h, 72 h ) were detected by quantitative-PCR.The expression of HVEM and LTβR on PBMC in patients with severe hepatitis were measured by flow cytometry.Results:In the MHV-3-induced murine fulminant hepatitis model ,liver injury in LIGHT KO mice was obviously decreased than that of WT mice,and ALT levels was also significantly lower than that of WT mice (P<0.01).The mRNA of HVEM and LTβR in the spleen were increased significantly after 48 h postinfection with MHV-3 ( P<0.05 );The level of LTβR mRNA in liver was significantly up-regulated in 12 h postinfection with MHV-3(P<0.01).Compared to healthy volunteers,the expression of both HVEM and LTβR on PBMC in patients with severe hepatitis was remarkably enhanced .Conclusion: TNFSF14 and its receptors LTβR and HVEM play a critical role in the pathogenesis of viral fulminant hepatitis .关键词
TNFSF14/LTβR/HVEM/病毒性肝炎Key words
TNFSF14/LTβR/HVEM/Viral hepatitis分类
医药卫生引用本文复制引用
李桂清,尚宇航,曹朝晖,杨菲,郑权友,王庆红,许桂莲..TNFSF14及其受体LTβR和HVEM在病毒肝炎中的作用[J].中国免疫学杂志,2015,31(12):1591-1594,4.基金项目
本文受国家自然科学基金面上项目(31270929)和重庆市自然科学基金面上项目(cstc2013jcyjA10129)资助. （31270929）
