中国免疫学杂志2015，Vol.31Issue(12)：1616-1620,5.DOI:10.3969/j.issn.1000-484X.2015.12.007
表皮生长因子对食管鳞癌细胞Eca109细胞周期及其调控因子的影响
Effects of epidermal growth factor on cell cycle and cell cycle-related regulatory factors of human esophageal squamous cell carcinoma cell line Eca109
摘要
Abstract
Objective:To investigate the effects of epidermal growth factor (EGF)on cell cycle and cell cycle-related regulatory factors of human esophageal squamous cell carcinoma (ESCC) cell line Eca109.Methods: Serum starved Eca109 cells were treated with 20 ng/ml recombinant human EGF(rhEGF)for 24 h.The cell cycle phase distribution was detected by flow cytometry.The mRNA and protein expression levels of p21CIP1/WAF1(p21) and p27KIP1(p27) were detected by real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)and Western blot,respectively.Results: The proportions of G1 phase cells in EGF group and control group were ( 54.90 ±0.82 )% and ( 65.94 ±0.74 )%.The mRNA and protein expression levels of p 21 in EGF group was significantly higher ,and p27 was significantly lower than that in control group ( P<0.01 ) .Conclusion: EGF facilitates G1-S phase transition,and promotes the proliferation of Eca 109 cells,which may be associated with the up-regulation of p21 and down-regulation of p27.关键词
表皮生长因子/食管鳞癌/Eca109细胞/细胞周期/p21CIP1/WAF1/p27KIP1Key words
Epidermal growth factor/Esophageal squamous cell carcinoma/Eca109 cell line/Cell cycle/p21CIP1/WAF1/p27 KIP1分类
医药卫生引用本文复制引用
李倩倩,朱红,王朝莉,黎仕娟,胡为民..表皮生长因子对食管鳞癌细胞Eca109细胞周期及其调控因子的影响[J].中国免疫学杂志,2015,31(12):1616-1620,5.基金项目
本文受四川省科技厅重点科技自筹项目(No.2011JYZ023)和川北医学院科研发展基金重点项目(No.CBY12-A-ZD19)资助. （No.2011JYZ023）
