中国免疫学杂志2015，Vol.31Issue(12)：1668-1673,6.DOI:10.3969/j.issn.1000-484X.2015.12.018
米诺地尔多克隆抗体的制备及其酶联免疫检测方法的建立
Preparation of polyclonal antibody for minoxidil, development of an ELISA method for detection of minoxidil
魏彪 1张癸荣 1张丹参 2聂凌云 3唐颜苹1
作者信息
- 1. 总后勤部卫生部药品仪器检验所,北京 100071
- 2. 河北北方学院,张家口 075000
- 3. 河北科技大学,石家庄 050018
- 折叠
摘要
Abstract
Objective:On the basis of synthetic antigens on minoxidil ,we prepared its polyclonal antibody and established a ELISA method to detect minoxidil .Methods:We synthesized minoxidil artificial antigen with glutaraldehyde and identified by UV scan -ning.We had established a detection of minoxidil competition ELISA .Results: The UV scan showed that the minoxidil successfully coupled to a carrier protein.The conjugate of minoxidil-BSA was used to immunize BALB/c mice.And the produced antiserum showed high titer of 1:12 800 in the indirect ELISA.Its ELISA curve equation:y=-0.082 3 x+0.938 ( R2 =0.9811 ) , minoxidil mass concentration and the absorbance in 0.001-10 μg/ml showed a good linearity .Conclusion: This method has been successfully preparing minoxidil artificial antigen and polyclonal antibodies and established ELISA detection method for minoxidil ,which provides a basis for the practical application of minoxidil immunoassay method .关键词
米诺地尔/人工抗原/多克隆抗体/ELISAKey words
Minoxidil/Artificial antigen/Polyclonal antibody/ELISA分类
医药卫生引用本文复制引用
魏彪,张癸荣,张丹参,聂凌云,唐颜苹..米诺地尔多克隆抗体的制备及其酶联免疫检测方法的建立[J].中国免疫学杂志,2015,31(12):1668-1673,6.
