草业学报Issue(12)：74-82,9.DOI:10.11686/cyxb2015051
立枯丝核菌对马铃薯侵染过程的显微结构观察与胞壁降解酶活性的测定
Pathogenic mechanism ofRhizoctonia solani potato blightⅠMicro-structure observation of the infection process and measurement of cell wall degradation enzyme activity
摘要
Abstract
Stem canker (black scurf disease)caused by Rhizoctonia solani has become one of the main soil-borne diseases which limit the development of the potato industry in Gansu province.In this study,R.solani spores were inoculated onto the stem base of potato plants grown in pots.The infection process and activity of potato cell wall degradation enzymes was observed and measured.Results showed that the infection sequence was bud,stem,root,stolon and lastly tuber.Hyphae attached to the surface of the host within 12 h,appressoria formed in 36 h,infection cushions formed in 48 h.Deformation and fracture of the cell wall,damage to the cell membrane,and the effects of plasmolysis such as deformation and fracture of the cell wall,damage to the cell and plastid membranes and the dissolution of cytoplasm were then observed under the electron microscope.The activities of cellulase (Cx),polygalacturonase (PG)and polymethyl-galacturonase (PMG)were 1.7,2.9 and 3.1 μg/(h·g),respectively,and these values were significantly higher than those of the control group.Theactivities of PG and PMG were as twice those of the control group.The increased enzyme activity may be relat-ed to tissue necrosis.关键词
立枯丝核菌/侵染过程/超微结构/透射电镜/胞壁降解酶Key words
Rhizoctonia solani/infection process/ultrastructure/transmission electron microscope/cell-wall-degrading enzymes引用本文复制引用
拓宁,张君,邱慧珍,张文明,张春红,刘星,朱静..立枯丝核菌对马铃薯侵染过程的显微结构观察与胞壁降解酶活性的测定[J].草业学报,2015,(12):74-82,9.基金项目
国家自然科学基金（31360500），国家公益性行业（农业）科研专项（201103004）和国家科技支撑计划项目（2012BAD06B03）资助。 （）
