南方医科大学学报Issue(12)：1665-1671,7.DOI:10.3969/j.issn.1673-4254.2015.12.01
疏水蛋白HFBI融合标签能显著提高外源基因在植物中的表达水平
Effect of the hydrophobin HFBI-fusion tag on exogenous protein accumulation in tobacco plant
摘要
Abstract
Objective To explore the mechanisms by which HFBI fusions increase recombinant fusion protein accumulation in plants. Methods The HFBI sequence from Trichoderma reesei was synthesized and two plant expression vectors for expression of green fluorescence protein (GFP) and GFP-HFBI were constructed. The vectors were inoculated in Nicotiana benthamiana plants through agroinfiltration, and the expression levels and mRNA accumulation levels of GFP in Nicotiana leaves were examined by Western blotting, ELISA and RT-PCR. Results The HFBI fusion tag significantly enhanced the accumulation of GFP in the leaves of N. benthamiana without causing toxic effects. Endoplasmic reticulum-targeted GFP-HFBI fusion induced the formation of spherical protein particles in the plant cells. Conclusion HFBI fusions can increase the accumulation of its fusion partner in plants by forming stable protein particles, which probably shields the target protein from endogenous protease-induced degadation. HFBI fusion technology provides an alternative to improving recombinant protein expression in plants from agroinfection-compatible expression vectors.关键词
真菌疏水蛋白/融合标签/绿色荧光蛋白/瑞氏木霉/本明烟Key words
hydrophobin/fusion tags/green fluorescent protein/Trichoderma reesei/Nicotiana benthamiana引用本文复制引用
张西倩,牟红珍,马婷,丁向真,李志英,王盛..疏水蛋白HFBI融合标签能显著提高外源基因在植物中的表达水平[J].南方医科大学学报,2015,(12):1665-1671,7.基金项目
国家自然科学基金（31160032）Supported by National Natural Science Foundation of China (31160032) （31160032）
