井冈山大学学报（自然科学版）Issue(6)：29-35,7.DOI:10.3969/j.issn.1674-8085.2015.06.007
邻香草醛缩胱氨酸双Schiff碱的合成及其对粟酒裂殖酵母细胞生长代谢的热动力学研究
SYNTHESIS AND THERMOKINETIC STUDY ON THE S.POMBE GROWTH METABOLISM OF A NOVEL BIS-SHCIFF BASE DERIVED FROM O-VANILLIN AND L-CYSTINE
摘要
Abstract
A novel Schiff base named Bis{2-[(3-mercapto propionic acid sodium-2-ylimino)-methyl]-6- methoxy-phenol}(abbreviated as OVCS) was synthesized using 2:1 molar ratio ofo-vanillin (C8H8O3) and L-cystine (C6H12N2O4S2). Elemental analysis, IR and NMR spectroscopy were employed to characterize the composition and structure of OVCS. The analytical results show that the molecular formula of the Schiff base is Na2(C22H22N2O8S2). The thermokinetic properties of the action of OVCS on the growth metabolism of Schizosaccharomyces pombe were investigated by a TAM air isothermal calorimetry at 305.15 K. Some thermokinetic parameters of growth metabolism ofS. pombe, such as the maximum heating power (Pmax),growth rate constant (k), generation time (tG), inhibition rate (I) and half inhibitory concentration (CI, 50) were calculated. Experimental results indicate that the values ofk, Qtotal andPmax ofS. pombe decrease, while the value ofI,tmax andtG ofS. pombe increased with the increase of the concentrations of OVCS. TheCI, 50value of OVCS was found to be 35.99 mg/L (or 9.62×10-2mol/L). From this work, one can reach a conclusion that OVCS possesses inhibitory effect on the growth metabolism ofS. pombe, and the greater the concentration, the stronger the inhibition.关键词
邻香草醛/L-胱氨酸/热动力学参数/粟酒裂殖酵母细胞Key words
o-vanillin/L-cystine/thermokinetic property/Schizosaccharomyces pombe cell分类
化学化工引用本文复制引用
蒋建宏,李强国,李旭,谷惠文,肖圣雄,胡钞粟,肖碧源,汤蒋莹,王文轩,邓媚莹..邻香草醛缩胱氨酸双Schiff碱的合成及其对粟酒裂殖酵母细胞生长代谢的热动力学研究[J].井冈山大学学报（自然科学版）,2015,(6):29-35,7.基金项目
国家自然科学基金项目(21273190)；湖南省科技计划项目(2013FJ3033,2012TP4021-6,2010FJ3053)；湘南学院科研基金项目(2013YJ36；2014XJ52)；湘南学院大学生研究性学习和创新性实验计划项目(校发[2013]15号) （21273190）
