检验医学与临床Issue(z2)：1-4,4.DOI:10.3969/j.issn.1672-9455.2015.26.001
ILK在肾间质纤维化过程中的表达及其干预效果的评价
The expression of ILK in the process of renal interstitial fibrosis and its intervention effect
摘要
Abstract
Objective To investigate the expressions and significances of ILK involved in PKB ,α‐SMA and E‐cadherin in the process of renal interstitial fibrosis and the effect of giving PI3K inhibitors (wortmannin) so as to provide a clue for clinical diagnosis and treatment .Methods 96 juvenilly female SD rats of 2 ~ 3 weeks were selected as experimental animals and made by unilateral ureteral ligation (UUO ) for renal interstitial fibrosis model .Rats were randomly divided into three groups as control group (group A ) ,UUO untreated group (group B) and UUO treated group (group C) .The expressions of ILK ,PKB ,α‐SMA and E‐cadherin of left kidney tissues were detected by immunohistochemical staining in 1w ,2w ,3w ,4w after model establishment .All datas of experiment were analyzed by statistics software SPSS17 .0 .Results The expressions of ILK ,PKB and α‐SMA in Group B were on the rise as the continuation of the experiment time and the differences were statistically significant at each time point compared with group A(P< 0 .05) .The expressions of E‐cadherin in Group B were on downward trend ,and the differences were sta‐tistically significant at each time point compared with group A(P< 0 .05) .The expressions of ILK ,PKB and α‐SMA in Group C raised significantly slowly as the continuation of the experiment time and the differences were statistically significant at each time point compared with group B(P< 0 .05) .The differences of the expressions of ILK and PKB in Group C were statistically significant compared with group A in 2w ,3w and 4w (P< 0 .05) .The expressions of E‐cadherin in Group C cut significantly slowly as the continuation of the experiment time and the differences were sta‐tistically significant compared with group B and A in 2w ,3w and 4w (P < 0 .05) .In group B ,the expression of ILK were positively correlated with the expression of PKB and α‐SMA by correlation analysis (P< 0 .05) ,and negatively correlated with the expression of E‐Cadherin (P< 0 .05) .Conclusion ILK is involved in the occurrence and develop‐ment of renal interstitial fibrosis .Giving PI3K inhibitors (wortmannin) can delay the progress of kidney disease ,and is expected to provide new targets for clinical treatment .关键词
整合素连接激酶/蛋白激酶 B/α-平滑肌蛋白/E-钙粘蛋白/肾间质纤维化Key words
ILK/PKB/α-SMA/E-cadherin/RIF引用本文复制引用
李小琴,马宏,郑玲芳..ILK在肾间质纤维化过程中的表达及其干预效果的评价[J].检验医学与临床,2015,(z2):1-4,4.基金项目
本课题为西安医学院第二附属医院科研基金资助项目（No ．11RFYFC04）。 （）
