检验医学与临床Issue(z2)：9-13,5.DOI:10.3969/j.issn.1672-9455.2015.26.004
伊立替康毒副作用预测基因UGT1A1多态性三种检测方法的比较研究
Comparison of three methods for the genotype of irinotecan toxicity prediction UGT1A1 gene
摘要
Abstract
Objective Using three method of fluorescence quantitative PCR ,PCR‐LDR and direct sequencing . we compared the genotyping of UGT1A1 * 28 ,UGT1A1 * 6 ,UGT1A1 * 93 and UGT1A1 * 60 four loci of UGT1A1for 45 cases of peripheral blood of tumor patients .Methods The fluorescence quantitative PCR ,PCR‐LDR and direct sequencing were used to detect the polymorphisms of UGT1A1 gene four loci .Results It's consistent for the result of four loci genotyping in 45 patients by the three methods .Conclusion Three detection methods have ad‐vantages and disadvantages respectively .We can choose the appropriate method according to the specific needs of the experiment .Among them ,with the characteristic of high detection sensitivity ,time saving ,simple operation and avoi‐ding the pollution the method of fluorescence quantitative PCR is suitable for clinical detection .关键词
UGT1A1/基因多态性/荧光定量 PCR/PCR-LDR/DNA 直接测序Key words
UGT1A1/gene polymorphism/fluorescence quantitative PCR/PCR-LDR/DNA direct sequencing引用本文复制引用
蔡利娟,冯孟秋,刘艳艳,卞如如,陈杰,陈疆荣,杨香山,刘玲..伊立替康毒副作用预测基因UGT1A1多态性三种检测方法的比较研究[J].检验医学与临床,2015,(z2):9-13,5.基金项目
山东省医学科学院科技项目（2013-015）。＃ （）
