检验医学与临床Issue(z2)：43-44,45,3.DOI:10.3969/j.issn.1672-9455.2015.26.018
浙江地区丙型肝炎患者的丙型肝炎病毒基因分型研究
Genotyping of hepatitis C virus obtained from patients in Zhejiang area
摘要
Abstract
Objective To investigate the prevalence of different genotypes of hepatitis C virus (HCV) in Zhe‐jiang Province ,and then to provide information for the diagnosis and prevention of HCV .Methods NS5B region of HCV from 365 serum samples was amplified by RT‐PCR .PCR products were then purified followed by sequencing . The sequences of these PCR products were compared with the Genebank sequences to construct the HCV phyloge‐netic tree and to determine HCV genotypes .Results 365 serum samples from HCV infected patients were examined . Six different HCV genotypes namely 1b ,2a ,2i ,3a ,3b and 6a were identified .The frequency of distribution of the gen‐otype 1b ,2a ,2i ,3a ,3b and 6a was 67 .7% ,20 .5% ,3% ,2 .7% ,4 .4% and 1 .6% ,respectively .Conclusion HCV 1b is the major genotype of patients distributed in Zhejiang Province .The distribution of HCV genotypes in Zhejiang is similar to those in other regions of China .关键词
丙型肝炎病毒/测序/基因分型Key words
hepatitis C virus/sequencing/genotype引用本文复制引用
陈奕磊,孙翠莲,王淑一..浙江地区丙型肝炎患者的丙型肝炎病毒基因分型研究[J].检验医学与临床,2015,(z2):43-44,45,3.
