检验医学与临床Issue(z2)：55-57,3.DOI:10.3969/j.issn.1672-9455.2015.26.024
ELISA法检测抗HBc和抗HBe的性能研究
Performance evaluation of anti HBc and anti HBe by ELISA testing
安哲 1李思鹏 1张妮 1李妙羡 1王香玲1
作者信息
- 1. 西安交通大学医学院第二附属医院 710004
- 折叠
摘要
Abstract
Objective To evaluate the performance of antiHBc and antiHBe tested by ELISA .Methods HBV‐M were tested by ELISA initially and retested by CMIA to evaluate the performance including Sensitivity ,Specificity , positive predictive value ,negative predictive value ,true positive rate and false negative rate et al .Results Se ,Sp ,PV+ ,PV‐of antiHBc and antiHBe by ELISA were 0 .76 ,0 .88 ,0 .99 ,0 .22 and 0 .77 ,0 .73 ,0 .86 ,0 .58 ,respectively ;true positive rate of antiHBc and antiHBe were 1 .00 and 0 ,99 in antiHBc(+ )/antiHBe( + ) subgroup ;in antiHBc( + )/antiHBe( - ) subgroup the true positive rate of antiHBc was 0 .98 and true negative rate of antiHBe was 0 .59 ;in an‐tiHBc( - )/antiHBe(+ ) subgroup true negative rate of antiHBc was 0 .22 and true positive rate of antiHBe was 0 . 82 .Conclusion ELISA for antiHBc has high specificity and low sensitivity which means high true positive rate and lots of false negative results ;ELISA for antiHBe has false positive and false negative results because of its low sensi‐tivity and specificity ;there are high accuracy for positive antiHBe with positive antiHBc and half of negative antiHBe with positive antiHBc were confirmed positive .Positive antiHBe with negative antiHBc has suggestive value for pre‐vious HBV infection .关键词
抗HBc/抗 HBe/酶联免疫吸附法/化学发光微粒子免疫测定/性能评价Key words
anti HBc/anti HBe/enzyme-linked immunosorbent assay(ELISA)/chemiluminecence micro-particle immunoassay(CMIA)/performance evaluation引用本文复制引用
安哲,李思鹏,张妮,李妙羡,王香玲..ELISA法检测抗HBc和抗HBe的性能研究[J].检验医学与临床,2015,(z2):55-57,3.
