山东农业大学学报(自然科学版)Issue(6):870-873,4.DOI:10.3969/j.issn.1000-2324.2015.06.014
五华鸡J亚群禽白血病的初步净化
Preliminary Eradication for Avian leukosis Virus- J Subgroup in Wuhua Chicken
摘要
Abstract
Avian leukosis virus (ALV) is the most common oncogenetic retrovirus that emerges spontaneously as a result of recombination between exogenous viruses, exogenous viruses and endogenous viruses, and exogenous viruses and non-homologous cellular genes. While there are no effective commercialized vaccines, pathogen eradication might be more operational. Parental group from Fanchang Wuhua breed conservation station were used for pathogen eradication, 5 batches with a total of 3059 blood samples were collected during July 2012 to April 2013. In addition, 1262 anal swab samples from 1 day old chicks were also collected. Antigen and antibody of ALV-J from blood samples were screened using ELISA method. Anal swab samples were also detected using ALV p27 antigen detection kit. The results showed that positive ALV-J in parental group was high to 40%. ALV-J positive rate to the four batches was 9.09%、5.53%、1.42% and 1.30%, respectively. In conclusion, the ALV-J positive rate was controlled in a very low percentage in parental group after this eradication process. These results could be meaningful for the further eradication of ALV-J.关键词
五华鸡/J 亚群禽白血病/初步净化Key words
Wuhua chicken/Avian leukosis virus J-subgroup/preliminary eradication分类
农业科技引用本文复制引用
李柔,盛昌树,徐前明,陈兴勇,耿照玉..五华鸡J亚群禽白血病的初步净化[J].山东农业大学学报(自然科学版),2015,(6):870-873,4.基金项目
安徽省科技攻关项目(12010302058) (12010302058)