世界中医药Issue(11)：1755-1759,5.DOI:10.3969/j.issn.1673-7202.2015.11.032
不同浓度蜜炙紫菀水煎剂对大肠癌 LOVO 细胞增殖和周期的影响
Effects of Honey-fried Radix Asteris Decoction(HFRAD)on Cell Proliferation and Cell Cycle of Colorectal Cancer LOVO Cells
摘要
Abstract
Objective:To explore the efficacy honey-fried Radix asteris decoction (HFRAD)of different concentration on cell cy-cle and proliferation of colorectal cancer LOVO cells.Methods:The changes of cellular morphology of LOVO cells treated with dif-ferent concentration HFRAD were observed by optical microscopy.MTT colorimetric assay was applied to observe proliferation of LOVO cells;flow cytometry was used to analyze cell cycle of LOVO cells.Results:MTT results indicated that proliferation of LO-VO cells treated with low concentration HFRAD(≤20mg·mL -1 )has been obviously promoted,and the cell proliferation rate could be up to 99.7%.Moreover,normal cellular morphology and membrane structure were observed by optical microscopy.Com-pared with the low concentration groups,it was found that cell proliferation of LOVO cells treated with high concentration HFRAD (>20mg·mL -1 )was obviously inhibited,the cell inhibitor rate can be up to above 80.34%.Optical microscopy results indica-ted that LOVO cells treated with high concentration HFRAD had lowest cell viability and the number of normal morphology cells can be significantly reduced.Flow cytometry results showed that the proportions of G1,S and G2 phase cells vary from different concentration HFARD and were significantly different from the control group.The proportion of S phase LOVO cells increased when treated with 10 and 20mg·mL -1 HFRAD.However,it was also found that the proportion of S phase LOVO cells decreased and the proportion of G2 phase increased with 30mg·mL -1 HFRAD.Conclusion:Different concentrate HFRAD has distinct effi-cacy on cell proliferation and cell cycle of LOVO cells.Cell proliferation of LOVO cells can be significantly promoted in concentra-tion of HFRAD(≤20mg·mL -1 ).However,high concentration HFRAD(>20mg·mL -1 )can inhibit cell proliferation.Moreo-ver,inhibition of G2 phase might be due to high concentration HFRAD.Our findings can provide experimental evidences for HFRAD in clinical therapy of Colorectal cancer.关键词
大肠癌LOVO细胞/蜜炙紫菀水煎剂/细胞增殖/细胞周期Key words
Colorectal cancer LOVO cells/Honey-fried Radix asteris decoction (HFRAD)/Cell proliferation/Cell cycle分类
医药卫生引用本文复制引用
杨映映,秦甜甜,钱树树,彭林,刘莉嘉,白松棉,王如峰,蒋燕,胡秀华..不同浓度蜜炙紫菀水煎剂对大肠癌 LOVO 细胞增殖和周期的影响[J].世界中医药,2015,(11):1755-1759,5.基金项目
国家级大学生创新创业训练计划课题---基于“肺与大肠相表里”理论研究紫菀及其提取物对大肠癌细胞增殖和迁移的影响（编号20131002602）；国家自然基金面上项目 （）
