色谱Issue(12)：1307-1313,7.DOI:10.3724/SP.J.1123.2015.07010
原位聚合法制备碳十八甲基丙烯酸酯聚合物及其用作开管毛细管电色谱固定相
Preparation of octadecyl methacrylate-based polymer stationary phase by in-situ polymerization for open tubular capillary electrochromatography
摘要
Abstract
The preparation of porous polymethacrylate-based open tubular capilary columns by in-situ copolymerization of octadecyl methacrylate ( OMA ), and ethylene dimethacrylate ( EDMA)in N,N-dimethylformamide( DMF)solvent are proposed. The parameters of the prep-aration procedure of the stationary phase are discussed in detail. The surface of the cross-section of an open tubular( OT)column by scanning electron microscope( SEM)showed wrin-kle configuration,which is expedient to enhance proportion of stationary phase and column capacity. Efficient separation of six alkyl benzene homologous series( namely,toluene,ethyl-benzene,propylbenzene,butylbenzene,amylbenzene and hexylbenzene ) were performed by CEC using the prepared column,and their elution order was in agreement with their hydropho-bicity. So,the OT columns bonded with octadecyl ligands yielded reversed-phase retention behavior toward nonpolar solutes. In addition,the separation of four antiepileptics was also investigated with satisfactory effectiveness. The column efficiency range was 35 300-49 800 plates/m. The results showed that the C18-OT column of organic matrix for reversed-phase chromatography was successfully prepared via in-situ polymerization in this work. Hereby ,this new column thus offers a promising new alternative in OT-CEC and will be useful in separation science.关键词
原位聚合/固定相/十八碳醇甲基丙烯酸酯/开管毛细管电色谱/抗癫痫药Key words
in-situ polymerization/stationary phase/octadecyl methacrylate( OMA )/open tubular capillary electrochromatography( OT-CEC)/antiepileptics分类
化学化工引用本文复制引用
周孙英,陈继涢,谭静静,林旭聪,谢增鸿..原位聚合法制备碳十八甲基丙烯酸酯聚合物及其用作开管毛细管电色谱固定相[J].色谱,2015,(12):1307-1313,7.基金项目
福建省自然科学基金项目（2013J01037）． （）
