武警医学2015，Vol.26Issue(12)：1224-1227,4.
缬沙坦抑制内质网应激对糖尿病肾损害大鼠足细胞的保护作用
Protective effect of valsartan on glomerular podocyte apoptosis induced by endoplasmic reticulum stress in diabetic nephropathy rats
摘要
Abstract
Objective To evaluate the effect of valsartan on apoptosis of podocytes ,GRP78 which serve as a symbolic protein of endoplasmic reticulum stress (ERS) and caspase12 in diabetic rats.Methods Male SD rats were randomly divided into normal group and study group .The animal models with diabetes were established in study group by STZ intraperitoneal injection ,then the study group were randomly divided into model group and valsartan group .Water was aiministered daily by gavage to normal group and model group.Valsartan was administered daily by gavage to valsartan group for 6 weeks.The levels of protein of nephrin、GRP78,caspase 12.BG,Cr ,BUN and 24-hour urinary protein quantity were checked .Results At the time of 6 weeks,compared with those in normal group,the number of apoptosis cells were much higher in the model kidneys ,the expression of GRP78(6.75 ±0.65),caspase 12 (11.51 ±0.32)in the model kidneys were much higher than GRP78(1.57 ±0.39),caspase12(2.66 ±0.57)in the normal group(P<0.05);however, the expression of nephrin in the model group (2.29 ±0.15)was lower than in the normal group (5.71 ±0.53)(P<0.05).While in the valsartan group,reductions were found in the expression of GRP78(3.14 ±1.00),caspase12 (8.08 ±2.48)and the number of apoptotic cells(P<0.05),the expression of nephrin (3.35 ±0.40)was higher(P<0.05).Conclusions Valsartan can depress the effect of endoplasmic reticulum stress and reduce the apotosis of the podocytes in the development of diabetic nephropa -thy.关键词
糖尿病肾病/内质网应激/足细胞凋亡/nephrin蛋白/缬沙坦Key words
diabetic nephropathy/endoplasmic reticulum stress/apoptosis/nephrin/valsartan分类
临床医学引用本文复制引用
魏静,张建荣..缬沙坦抑制内质网应激对糖尿病肾损害大鼠足细胞的保护作用[J].武警医学,2015,26(12):1224-1227,4.基金项目
国家自然科学基金面上项目(81273706) （81273706）
