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鸡卵清提取液不同成分对细胞周期与细胞凋亡的影响

阮光萍 姚翔 刘菊芬 王金祥 庞荣清 潘兴华

中国组织工程研究Issue(46):7451-7455,5.
中国组织工程研究Issue(46):7451-7455,5.DOI:10.3969/j.issn.2095-4344.2015.46.014

鸡卵清提取液不同成分对细胞周期与细胞凋亡的影响

Different components of chicken egg-white extracts affect cell cycle and apoptosis

阮光萍 1姚翔 2刘菊芬 3王金祥 1庞荣清 2潘兴华3

作者信息

  • 1. 解放军成都军区昆明总医院细胞生物治疗中心,云南省昆明市 650032
  • 2. 干细胞与免疫细胞生物医药技术国家地方联合工程实验室,云南省昆明市 650032
  • 3. 云南省细胞治疗技术转化医学重点实验室,云南省昆明市 650032
  • 折叠

摘要

Abstract

BACKGROUND:If an extract can prolong the S phase and reduce the percentage of apoptosis after co-culture with cels, it is proved that the extract is able to promote cel proliferation. OBJECTIVE: To prove the effects of chicken egg-white extracts with different components on the proliferation, cel cycle and apoptosis of 293T cels. METHODS: An ultrafiltration unit was used to separate chicken egg-white extracts into different components that were > 10 ku, < 10 ku, > 3 ku and < 3 ku to co-culture with cels for 3 days. Then, cel proliferation, cel cycle and cel apoptosis were detected. RESULTS AND CONCLUSION:Chicken egg-white extract components of < 10 ku and < 3 ku could promote cel proliferation, increase the percentage of S-phase cels and reduce the percentage of apoptosis. In conclusion, chicken egg-white extract components that are < 10 ku and < 3 ku can promote cel proliferation, as wel as increase the percentage of S-phase cels and reduce apoptosis percentage.

关键词

组织构建/组织工程/鸡卵清提取液/不同成分/细胞周期/细胞凋亡/细胞增殖

分类

医药卫生

引用本文复制引用

阮光萍,姚翔,刘菊芬,王金祥,庞荣清,潘兴华..鸡卵清提取液不同成分对细胞周期与细胞凋亡的影响[J].中国组织工程研究,2015,(46):7451-7455,5.

基金项目

国家支撑计划(2014BAI01B01);云南省科技计划项目(2013CA005)资助的课题@@@@the National Support Program of China, No.2014BAI01B01 (2014BAI01B01)

Yunnan Provincial Science and Technology Project, No.2013CA005 ()

中国组织工程研究

OA北大核心CSTPCD

2095-4344

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