摘要
Abstract
Objective To investigate the effects of adipose-derived stem cell(ADSC) on the Neurocan expression in rat with cerebral ischemia. Methods 54 clean class adult male Sprague-Dawley rats were selected and randomly divided into the sham operation group,model group(middle cerebral arterial embolism) and ADSC group(ADSC transplantation after middle cere-bral arterial embolism),18 cases in each group. The middle cerebral arterial embolism model adopted the modified Zea-Longa line embolism method. Before ADSC transplantation,diamidino-2-phenylindole (DAPI) labeling was performed. The ADSC group was injected with ADSC(1×106 cells) by lateral ventricle on 1 d after successful model establishment. Rats were sacrificed for taking the brain on postoperative 7,14,28 d and the Neurocan protein expression was detected by using the immunofluorescence and Western blot. Results The Neurocan expression of the brain tissues on postoperative 7 ,14 d in the model group was significantly increased and showed the sheet irregular distribution,which was decreased on 28 d,but still higher than that in the sham operation group,the differences were statistically significant(P<0.05). The Neurocan expression of the brain tissues on postoperative 7,14 d in the ADSC group was significantly decreased,moreover which continued to 28 d and showed the statistical difference compared with the model group(P<0.05). Conclusion The ADSC transplantation can decrease the Neurocan expression in the brain ischemic tissues and reduce the scar generation ,which provides the enabling environment for late nerve axons maturity and function estab-lishment,and can promote the restoration of neural function.关键词
干细胞/疾病模型,动物/颅内栓塞和血栓形成/蛋白聚糖类/神经轴突Key words
Stem cells/Disease models,animal/Intracranial embolism and thrombosis/Proteoglycans/Neuronal axon
