中国农业科学Issue(19)：3857-3865,9.DOI:10.3864/j.issn.0578-1752.2015.19.008
橘小实蝇幼虫解毒酶系基因应对高效氯氰菊酯胁迫的组织特异性表达
Tissue Specific Expression of Genes Encoding Detoxification Enzymes in the Larvae ofBactrocera dorsalis Underβ-Cypermethrin Stress
摘要
Abstract
Objective]Based on the transcriptome data ofBactrocera dorsalis, the aim of this study is to systematically analyze the expression of genes in larvae encoding detoxification enzymes underβ-cypermethrin exposure, identify important detoxification genes, and focus on the discussion of biological significance of tissue specific expression of these genes.[Method] The expressions of 30 detoxification genes in the whole body of larvae, midgut, and fat body were detected underβ-cypermethrin exposure by using qPCR.[Result]Under the stress ofβ-cypermethrin, the qPCR results of 8 GSTs genes showed that the mRNA levels of 4 Delta class genes significantly increased. The expressions of genes from Delta class were up-regulated to 5.6-32.5 folds in the midgut and fat body, but the expressions of other GST genes did not show very active reaction when exposed toβ-cypermethrin. Meanwhile, although the expressions of Delta class GSTs genes significantly increased in midgut and fat body, the expression changes of these genes were not so apparent in the whole body. As for the 6 CCEs genes, their expressions were up-regulated in some degree in the whole body, andα-E3 was up-regulated to 4.1 folds. However, in the midgut and fat body, the expressions of these genes did not increase significantly. The qPCR results of 16 P450s genes showed that the expressions of most P450s genes were up-regulated, especially the genes from CYP4 and CYP6 families. In CYP4 family, the expressions ofCYP4D47 (midgut),CYP4E9(whole body and fat body), CYP4P5(whole body and fat body) were highly up-regulated. The expression ofCYP4AD1 increased in some degree in the midgut and fat body, but no significant changes were found in the whole body.CYP4S18andCYP4AC4 did not show any positive reaction or their expression changes were not apparent. In CYP6 family, the expressions ofCYP6A48 andCYP6A41 had a significant increase in the fat body, 18.0 and 9.6 folds up-regulated, respectively. The expression of others likeCYP6G6 and CYP6A50also increased. The expression ofCYP6EK1was up-regulated by 4.8 folds in the whole body, but did not change in midgut and fat body. The expressions of the other genes, such asCYP12C2,CYP12N1,CYP9F6, andCYP309B1 also significantly increased in the fat body when exposed toβ-cypermethrin. But the expression ofCYP317B1 did not change under the stress.[Conclusion]When under the stress ofβ-cypermethrin, the detoxification genes inB. dorsalis larvae were highly enriched in midgut and fat body, but showed a balance in the whole body. It suggests that when under the toxic stress, the pests may have special strategy for efficient use and proper distribution of energy, which increase the energy efficiency to decrease the extra energy cost to resist the damage of exogenous toxin. This kind of strategy might be an important acclimation of insects to avoid fitness cost when under the environmental pressures.关键词
橘小实蝇/GSTs/P450s/CCEs/药剂胁迫/组织特异性Key words
Bactrocera dorsalis/GSTs/P450s/CCEs/toxic stress/tissue specificity引用本文复制引用
申光茂,王晓娜,黄勇,豆威,王进军..橘小实蝇幼虫解毒酶系基因应对高效氯氰菊酯胁迫的组织特异性表达[J].中国农业科学,2015,(19):3857-3865,9.基金项目
国家公益性行业（农业）科研专项（201203038）、现代柑橘产业技术体系岗位科学家经费（CARS-27）、中央高校基本科研业务费团队项目 （）
