中山大学学报（自然科学版）Issue(6)：27-30,4.DOI:10.13471/j.cnki.acta.snus.2015.06.005
褐飞虱可溶性蛋白 SDS－PAGE 电泳分析
Soluble Proteins in Nilaparvata lugens (Stål) by SDS-PAGE
摘要
Abstract
The soluble proteins in Nilaparvata lugens adults and nymphs were analyzed by SDS-PAGE e-lectrophoresis.Results showed that there were almost equal number protein bands in all types of adult samples, most bands in different wing or gender samples existed content differences except female adult-specific protein band.There were four bands significantly higher in long-winged adult samples than in short-winged adult samples;in the comparison of male and female adult samples, it was found that there were two bands higher in males than in females, while other three bands higher in females than in males. The bands in nymph samples were gradually increased with the increase of age, four bands were constant-ly expressed in each instar period, one more band appeared in 5th instar nymph samples, and one band could be detected from the 4th instar.In addition, the contents of two bands were found much higher in long wing bud nymphs (9.029, 9.563 ng/μL) than those in short wing bud nymphs (5.133, 3.111 ng/μL) .Last we discussed the relationships among the different bands and wing dimorphism or sex differ-entiation in insects.关键词
SDS-PAGE/褐飞虱/可溶性蛋白/翅二型性Key words
SDS-PAGE/Nilaparvata lugens Stål/soluble proteins/wing dimorphism分类
生物学引用本文复制引用
梁梓强,梁士可,张梅,王方海..褐飞虱可溶性蛋白 SDS－PAGE 电泳分析[J].中山大学学报（自然科学版）,2015,(6):27-30,4.基金项目
国家自然科学基金资助项目（311771844）；广东省自然科学基金资助项目 （）
